Yoon Chi-Su, Ko Wonmin, Lee Dong-Sung, Kim Dong-Cheol, Kim Jongsu, Choi Moonbum, Beom Jin Seon, An Ren-Bo, Oh Hyuncheol, Kim Youn-Chul
College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea.
Department of Biomedical Chemistry, College of Health and Biomedical Science, Konkuk University, Chungju, Chungbuk 27478, Republic of Korea.
Mol Med Rep. 2017 Apr;15(4):2347-2352. doi: 10.3892/mmr.2017.6237. Epub 2017 Feb 22.
Taraxacum coreanum Nakai is a dandelion that is native to Korea, and is widely used as an edible and medicinal herb. The present study revealed the neuroprotective effect of this plant against glutamate-induced oxidative stress in HT22 murine hippocampal neuronal cells. Ethanolic extracts from the aerial (TCAE) and the root parts (TCRE) of T. coreanum were prepared. Both extracts were demonstrated, by high performance liquid chromatography, to contain caffeic acid and ferulic acid as representative constituents. TCAE and TCRE significantly increased cell viability against glutamate-induced oxidative stress in mouse hippocampal HT22 cells. Western blot analysis revealed that treatment of HT22 cells with the extracts induced increased expression of the enzyme heme oxygenase-1 (HO-1), compared with untreated cells, in a concentration-dependent manner. Increased HO-1 enzymatic activity, compared with untreated cells, was also demonstrated following treatment with TCAE and TCRE. In addition, western blot analysis of the nuclear fractions of both TCAE and TCRE-treated HT22 cells revealed increased levels of nuclear factor erythroid 2 like 2 (Nrf2) compared with untreated cells, and decreased Nrf2 levels in the cytoplasmic fraction compared with untreated cells. The present study suggested that the neuroprotective effect of T. coreanum is associated with induction of HO-1 expression and Nrf2 translocation to the nucleus. Therefore, T. coreanum exhibits a promising function in prevention of neurodegeneration. Further studies will be required for the isolation and the full characterization of its active substances.
朝鲜蒲公英是一种原产于韩国的蒲公英,被广泛用作食用和药用草本植物。本研究揭示了这种植物对HT22小鼠海马神经元细胞中谷氨酸诱导的氧化应激的神经保护作用。制备了朝鲜蒲公英地上部分(TCAE)和根部(TCRE)的乙醇提取物。通过高效液相色谱法证明,两种提取物均含有作为代表性成分的咖啡酸和阿魏酸。TCAE和TCRE显著提高了小鼠海马HT22细胞对谷氨酸诱导的氧化应激的细胞活力。蛋白质印迹分析显示,与未处理的细胞相比,用提取物处理HT22细胞以浓度依赖性方式诱导了血红素加氧酶-1(HO-1)的表达增加。与未处理的细胞相比,用TCAE和TCRE处理后也显示出HO-1酶活性增加。此外,对TCAE和TCRE处理的HT22细胞核部分的蛋白质印迹分析显示,与未处理的细胞相比,核因子红细胞2样2(Nrf2)水平增加,与未处理的细胞相比,细胞质部分的Nrf2水平降低。本研究表明,朝鲜蒲公英的神经保护作用与HO-1表达的诱导和Nrf2向细胞核的转位有关。因此,朝鲜蒲公英在预防神经退行性变方面具有有前景的功能。其活性物质的分离和全面表征还需要进一步研究。