He Mei Tong, Park Chan Hum, Shin Yu Su, Kim Ji Hyun, Cho Eun Ju
College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea.
Institute of New Frontier Research Team, Research Institute of Medical-Bio Convergence, Hallym University, Chuncheon 24252, Republic of Korea.
Foods. 2023 Sep 28;12(19):3617. doi: 10.3390/foods12193617.
Oxidative stress is closely associated with the pathology of neurodegenerative diseases. The seeds of L. (CTS) and (TC) are reported as herbal medicines for neuroprotection. This study investigated the protective effect of CTS, TC, and their combination against oxidative stress induced by HO in SH-SY5Y cells. The CTS and TC combination dose-dependently increased DPPH and ·OH radical scavenging activities compared with non-combination. The combination showed a higher increased cell survival rate in HO-stimulated SH-SY5Y cells than CTS or TC. Moreover, CTS, TC, and their combination-treated cells reduced LDH release and apoptotic cells. CTS, TC, and their combination also inhibited NO and ROS generation. Further, the combination of up-regulated antioxidant enzymes (superoxide dismutase and glutathione peroxidase) and Bcl-2 protein expressions and down-regulated Bax expression. These findings suggest that the combination of CTS and TC may be beneficial to prevent and treat oxidative stress-mediated neurodegenerative diseases.
氧化应激与神经退行性疾病的病理密切相关。长柄扁桃种子（CTS）和郁李仁（TC）被报道为具有神经保护作用的草药。本研究调查了CTS、TC及其组合对过氧化氢（HO）诱导的SH-SY5Y细胞氧化应激的保护作用。与未组合相比，CTS和TC组合剂量依赖性地增加了二苯基苦味酰基自由基（DPPH）和羟基自由基（·OH）的清除活性。该组合在HO刺激的SH-SY5Y细胞中显示出比CTS或TC更高的细胞存活率增加。此外，CTS、TC及其组合处理的细胞减少了乳酸脱氢酶（LDH）释放和凋亡细胞。CTS、TC及其组合还抑制了一氧化氮（NO）和活性氧（ROS）的产生。此外，该组合上调了抗氧化酶（超氧化物歧化酶和谷胱甘肽过氧化物酶）和Bcl-2蛋白表达，并下调了Bax表达。这些发现表明，CTS和TC的组合可能有利于预防和治疗氧化应激介导的神经退行性疾病。
