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HOX转录本反义RNA及上皮-间质转化相关因子在食管鳞状细胞癌中的表达及意义

The expression and significance of HOX transcript antisense RNA and epithelial-mesenchymal transition-related factors in esophageal squamous cell carcinoma.

作者信息

Da Chunli, Zhan Yiyi, Li Yu, Tan Yao, Li Ruiguang, Wang Ruozheng

机构信息

Department of Radiotherapy for Head and Neck, Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.

Department of Chemotherapy for Lung Cancer, Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.

出版信息

Mol Med Rep. 2017 Apr;15(4):1853-1862. doi: 10.3892/mmr.2017.6210. Epub 2017 Feb 16.

Abstract

The present study investigated the correlation and significance of HOX transcript antisense RNA (HOTAIR) and epithelial-mesenchymal transition (EMT)-related factors in the occurrence and metastasis of esophageal squamous cell cancer (ESCC) progression. The mRNA and protein expression levels of HOTAIR and EMT‑related factors were detected in 96 ESCC and para‑carcinoma tissues using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The expression levels of these factors, and the correlation between these factors and clinicopathological characteristics were subsequently analyzed. HOTAIR mRNA expression levels were significantly higher in ESCC compared with in para-carcinoma tissues, and HOTAIR mRNA expression levels were significantly higher in the groups with lymph node involvement or organ metastasis compared with the group without. Furthermore, HOTAIR expression levels demonstrated a significant increasing trend from well‑differentiated cancer to poorly differentiated cancer. The mRNA and protein expression levels of zinc finger protein SNAI1 (Snail) and β‑catenin in ESCC were significantly higher compared with para-carcinoma tissues, whereas E‑cadherin mRNA and protein expression levels were lower in ESCC tissues compared with in para-carcinoma tissues. Snail mRNA and protein expression levels were also significantly higher in groups with lymph node involvement or organ metastasis compared with those without, and β‑catenin protein expression levels were significantly higher in the groups with lymph node involvement or organ metastasis compared with the group without. In the 96 ESCC tissues, HOTAIR mRNA expression levels were positively correlated with Snail mRNA and protein expression levels, and were negatively correlated with E‑cadherin expression levels. HOTAIR mRNA expression levels were also positively correlated with β‑catenin mRNA expression levels. In conclusion, HOTAIR may be involved in carcinogenesis and metastasis, and may induce the expression of EMT‑related factors; detection of these factors may assist in early diagnosis and prognostic prediction.

摘要

本研究探讨了HOX转录反义RNA(HOTAIR)与上皮-间质转化(EMT)相关因子在食管鳞状细胞癌(ESCC)发生发展及转移中的相关性及意义。采用逆转录-定量聚合酶链反应和蛋白质印迹分析检测96例ESCC组织及癌旁组织中HOTAIR和EMT相关因子的mRNA和蛋白表达水平。随后分析这些因子的表达水平以及这些因子与临床病理特征之间的相关性。与癌旁组织相比,ESCC中HOTAIR mRNA表达水平显著升高,与无淋巴结受累或器官转移的组相比,有淋巴结受累或器官转移的组中HOTAIR mRNA表达水平显著更高。此外,从高分化癌到低分化癌,HOTAIR表达水平呈显著上升趋势。与癌旁组织相比,ESCC中锌指蛋白SNAI1(Snail)和β-连环蛋白的mRNA和蛋白表达水平显著更高,而ESCC组织中E-钙黏蛋白的mRNA和蛋白表达水平低于癌旁组织。与无淋巴结受累或器官转移的组相比,有淋巴结受累或器官转移的组中Snail的mRNA和蛋白表达水平也显著更高,且有淋巴结受累或器官转移的组中β-连环蛋白蛋白表达水平显著高于无淋巴结受累或器官转移的组。在96例ESCC组织中,HOTAIR mRNA表达水平与Snail的mRNA和蛋白表达水平呈正相关,与E-钙黏蛋白表达水平呈负相关。HOTAIR mRNA表达水平也与β-连环蛋白mRNA表达水平呈正相关。综上所述,HOTAIR可能参与致癌和转移过程,并可能诱导EMT相关因子的表达;检测这些因子可能有助于早期诊断和预后预测。

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