在亚核系统中启动海拉细胞DNA合成。
Initiation of HeLa cell DNA synthesis in a subnuclear system.
作者信息
Brun G, Weissbach A
出版信息
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5931-5. doi: 10.1073/pnas.75.12.5931.
Abstract
Mammalian cells are known to synthesize DNA in discrete stages, the first of which seems to be the formation of DNA pieces 150--200 nucleotides in length that have a s20 value of about 4 S. We have reconstructed a system derived from HeLa cell nuclei that carries out RNA-primed initiation of the synthesis of small (4S) DNA fragments. This synthesis is resistant to high concentrations of alpha-amanitin and sensitive to antibody directed against RNA polymerase I, suggesting that this enzyme may be involved in the initiation step. The formation of small DNA fragments in this system also requires DNA polymerase alpha, heat-labile nuclear factor(s), and at least one other nuclear protein.
摘要
已知哺乳动物细胞在不同阶段合成DNA，其中第一步似乎是形成长度为150 - 200个核苷酸的DNA片段，其沉降系数约为4S。我们构建了一个源自HeLa细胞核的系统，该系统能进行RNA引发的小（4S）DNA片段合成的起始反应。这种合成对高浓度的α - 鹅膏蕈碱有抗性，而对针对RNA聚合酶I的抗体敏感，这表明该酶可能参与起始步骤。此系统中小DNA片段的形成还需要DNA聚合酶α、热不稳定核因子以及至少一种其他核蛋白。
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