Lieberman M E, Maurer R A, Gorski J
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5946-9. doi: 10.1073/pnas.75.12.5946.
Primary cultures of rat pituitary cells respond to estradiol-17beta by increased incorporation of radiolabeled precursors into prolactin but not into the bulk of other cellular proteins. The rate of increase in prolactin synthesis is dose dependent, reaching maximal levels in the physiological range of estradiol. At a concentration of 1 nM, estradiol, diethylstilbestrol, and estriol are stimulatory whereas androgens, progesterone, and corticosterone are without significant effect. Exposure of pituitary cells to 10 nM estradiol resulted in a 500% increase in prolactin synthesis after 7 days of culture. The results indicate that estradiol can stimulate prolactin synthesis through direct action on the pituitary.
大鼠垂体细胞的原代培养物对17β-雌二醇有反应，表现为放射性标记前体掺入催乳素的量增加，而掺入其他细胞蛋白质总量的量未增加。催乳素合成的增加速率呈剂量依赖性，在雌二醇的生理范围内达到最高水平。在浓度为1 nM时，雌二醇、己烯雌酚和雌三醇具有刺激作用，而雄激素、孕酮和皮质酮则无显著影响。垂体细胞在培养7天后暴露于10 nM雌二醇，催乳素合成增加了500%。结果表明，雌二醇可通过对垂体的直接作用刺激催乳素合成。
