Nichols E J, Manger R, Hakomori S I, Rohrschneider L R
Fred Hutchinson Cancer Research Center, Division of Basic Sciences, Seattle, Washington 98104.
Exp Cell Res. 1987 Dec;173(2):486-95. doi: 10.1016/0014-4827(87)90288-6.
The effects of castanospermine on various parameters associated with transformation were examined in cells expressing the viral oncogene v-fms. Fischer rat embryo (FRE) cells transformed by the oncogene v-fms and grown in the presence of castanospermine reverted to a more normal cell morphology and accumulated fms protein within the endoplasmic reticulum. Treated cells attained contact inhibition of cell growth at a much lower cell density compared to the untreated controls. No effect of castanospermine on cell growth was observed for FRE cells transformed by a different oncogene v-fgr. Castanospermine-treated SM-FRE (v-fms transformed) cells reexpressed extracellular matrix fibronectin and exhibited an extensive actin-containing cytoskeleton similar to that of normal nontransformed FRE cells. Castanospermine treatment of SM-FRE cells resulted in a sixfold decrease in [3H]deoxyglucose uptake compared to that of the nonreverted SM-FRE cells. Again, no effect was observed in FRE cells transformed by the oncogene v-fgr (GR-FRE). These results further characterize the reversion caused by castanospermine and indicate that cell surface expression coordinately controls anchorage independent growth, cell morphology, contact inhibition of growth, and hexose uptake.
在表达病毒癌基因v-fms的细胞中，研究了栗精胺对与转化相关的各种参数的影响。由癌基因v-fms转化并在栗精胺存在下生长的Fischer大鼠胚胎（FRE）细胞恢复到更正常的细胞形态，并在内质网中积累fms蛋白。与未处理的对照相比，处理后的细胞在低得多的细胞密度下达到细胞生长的接触抑制。对于由不同癌基因v-fgr转化的FRE细胞，未观察到栗精胺对细胞生长的影响。经栗精胺处理的SM-FRE（v-fms转化）细胞重新表达细胞外基质纤连蛋白，并表现出与正常未转化FRE细胞相似的广泛的含肌动蛋白的细胞骨架。与未回复的SM-FRE细胞相比，栗精胺处理SM-FRE细胞导致[3H]脱氧葡萄糖摄取减少六倍。同样，在由癌基因v-fgr（GR-FRE）转化的FRE细胞中未观察到影响。这些结果进一步表征了栗精胺引起的回复，并表明细胞表面表达协同控制锚定非依赖性生长、细胞形态、生长的接触抑制和己糖摄取。
