Laboratory of Neural Signal Transduction, Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.
Laboratory of Neural Signal Transduction, Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Sci Rep. 2017 Mar 6;7:43865. doi: 10.1038/srep43865.
Sonic hedgehog (SHH) as a secreted protein is important for neuronal development in the central nervous system (CNS). However, the mechanism about SHH release remains largely unknown. Here, we showed that SHH was expressed mainly in the synaptic vesicles of hippocampus in both young postnatal and adult rats. High, but not low, frequency stimulation, induces SHH release from the neurons. Moreover, removal of extracellular Ca, application of tetrodotoxin (TTX), an inhibitor of voltage-dependent sodium channels, or downregulation of soluble n-ethylmaleimide-sensitive fusion protein attachment protein receptors (SNAREs) proteins, all blocked SHH release from the neurons in response to HFS. Our findings suggest a novel mechanism to control SHH release from the hippocampal neurons.
作为一种分泌蛋白，Sonic hedgehog（SHH）对于中枢神经系统（CNS）中的神经元发育非常重要。然而，SHH 释放的机制在很大程度上仍不清楚。在这里，我们发现 SHH 主要在幼年和成年大鼠的海马突触小泡中表达。高频率刺激而非低频率刺激可诱导神经元释放 SHH。此外，去除细胞外 Ca2+、应用电压门控钠通道抑制剂河豚毒素（TTX）或下调可溶性 N-乙基马来酰亚胺敏感融合蛋白附着蛋白受体（SNAREs）蛋白，均可阻断高频刺激引起的神经元 SHH 释放。我们的研究结果表明了一种控制海马神经元 SHH 释放的新机制。
