Department of Medical Microbiology, Virology Unit, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.
Turk J Med Sci. 2017 Feb 27;47(1):194-200. doi: 10.3906/sag-1508-155.
BACKGROUND/AIM: Human papillomaviruses have been established as a risk factor for invasive carcinoma of the uterine cervix. HPV E6/E7 oncogene expression has recently emerged as a promising biomarker to determine the risk for progression to high-grade cervical lesions. The aim of this study was to evaluate HPV mRNA and DNA detection in samples with abnormal cytology.
Cervical specimens were obtained at the Department of Obstetrics and Gynecology via cervical brushes during January-October 2011. Liquid-based cytology slides were evaluated according to the 2001 Bethesda System. Cytology specimens from a total of 81 women with abnormal cytology were included. Real-time PCR and NASBA assays were performed to detect HPV DNA and E6/E7 mRNA, respectively.
HPV DNA was identified in 73 samples (90.1%). HPV E6/E7 mRNA expression was observed in 45 samples (55.6%). A statistically significant difference was observed among cytological diagnosis groups. In 25 patients, a biopsy was performed during the follow-up. HPV DNA was detected in all of these patients. HPV E6/E7 expression was present only in CIN I-III diagnosed patients.
The E6/E7 mRNA test is a robust indicator of cytological atypia and correlates better with progressive lesions than DNA assays.
背景/目的：人乳头瘤病毒（HPV）已被确定为宫颈癌浸润性癌的危险因素。HPV E6/E7 致癌基因的表达最近已成为确定进展为高级别宫颈病变风险的有前途的生物标志物。本研究的目的是评估 HPV mRNA 和 DNA 在细胞学异常样本中的检测。
2011 年 1 月至 10 月，在妇产科通过宫颈刷收集宫颈标本。根据 2001 年 Bethesda 系统评估液基细胞学幻灯片。共纳入 81 例细胞学异常的女性的细胞学标本。分别使用实时 PCR 和 NASBA 检测 HPV DNA 和 E6/E7 mRNA。
73 例（90.1%）标本中检测到 HPV DNA。45 例（55.6%）标本中观察到 HPV E6/E7 mRNA 表达。细胞学诊断组之间存在统计学显著差异。在 25 例患者中，在随访期间进行了活检。这些患者均检测到 HPV DNA。仅在 CIN I-III 诊断患者中存在 HPV E6/E7 表达。
E6/E7 mRNA 检测是细胞学异常的有力指标，与 DNA 检测相比，与进展性病变的相关性更好。
