Zanacic Enisa, McMartin Dena W, Stavrinides John
a Engineering Support & Research, SaskWater, Moose Jaw, Regina, SK S6H 1C8, Canada.
b Faculty of Engineering and Applied Science, University of Regina, Regina, SK S4S 0A2, Canada.
Can J Microbiol. 2017 Jun;63(6):546-558. doi: 10.1139/cjm-2017-0077. Epub 2017 Mar 6.
Rural communities rely on surface water reservoirs for potable water. Effective removal of chemical contaminants and bacterial pathogens from these reservoirs requires an understanding of the bacterial community diversity that is present. In this study, we carried out a 16S rRNA-based profiling approach to describe the bacterial consortia in the raw surface water entering the water treatment plants of 2 rural communities. Our results show that source water is dominated by the Proteobacteria, Bacteroidetes, and Cyanobacteria, with some evidence of seasonal effects altering the predominant groups at each location. A subsequent community analysis of transects of a biological carbon filter in the water treatment plant revealed a significant increase in the proportion of Proteobacteria, Acidobacteria, Planctomycetes, and Nitrospirae relative to raw water. Also, very few enteric coliforms were identified in either the source water or within the filter, although Mycobacterium was of high abundance and was found throughout the filter along with Aeromonas, Legionella, and Pseudomonas. This study provides valuable insight into bacterial community composition within drinking water treatment facilities, and the importance of implementing appropriate disinfection practices to ensure safe potable water for rural communities.
农村社区依靠地表水库提供饮用水。要有效去除这些水库中的化学污染物和细菌病原体，需要了解其中存在的细菌群落多样性。在本研究中，我们采用基于16S rRNA的分析方法来描述进入两个农村社区水处理厂的原地表水中的细菌群落。我们的结果表明，水源以变形菌门、拟杆菌门和蓝细菌为主，有一些证据表明季节性影响会改变每个地点的优势菌群。随后对水处理厂生物碳滤池断面的群落分析显示，相对于原水，变形菌门、酸杆菌门、浮霉菌门和硝化螺旋菌门的比例显著增加。此外，在水源水或滤池中均未检测到肠道大肠菌群，不过分枝杆菌数量众多，在整个滤池中都能发现，同时还有气单胞菌、军团菌和假单胞菌。本研究为饮用水处理设施中的细菌群落组成提供了有价值的见解，并强调了实施适当消毒措施以确保农村社区安全饮用水的重要性。
