从啮齿动物细小病毒——基尔汉姆大鼠病毒中分离并鉴定热敏突变体。
Isolation and characterization of thermosensitive mutants from Kilham rat virus, a rodent parvovirus.
作者信息
Wicker R, Gunther M
机构信息
Institut Curie, Section de Biologie, Paris, France.
出版信息
J Gen Virol. 1988 Jan;69 ( Pt 1):163-75. doi: 10.1099/0022-1317-69-1-163.
Thermosensitive mutants were isolated from nitrous acid-treated Kilham rat virus (KRV). At a restrictive temperature (39.5 degrees C), the mutants tested did not produce appreciable amounts of infectious particles, haemagglutinin or progeny single-stranded DNA. Virus antigen accumulation as detected by immunoperoxidase was reduced. Complementation tests revealed two distinct groups. The three members of complementation group 1 synthesized normal amounts of replicative forms but were restricted in single-stranded DNA production and capsid protein expression, exhibiting phenotypes compatible with cap mutations. The sole representative of group 2, KRV ts6, failed to accumulate replicative forms, displaying a rep- phenotype. These mutants provide new tools to test the role of viral products in the biology of autonomous parvoviruses.
从经亚硝酸处理的基尔汉姆大鼠病毒（KRV）中分离出热敏突变体。在限制温度（39.5摄氏度）下，所测试的突变体不会产生大量有感染性的颗粒、血凝素或子代单链DNA。通过免疫过氧化物酶检测到的病毒抗原积累减少。互补试验揭示了两个不同的组。互补组1的三个成员合成了正常量的复制形式，但在单链DNA产生和衣壳蛋白表达方面受到限制，表现出与帽突变相容的表型。组2的唯一代表KRV ts6未能积累复制形式，表现出rep-表型。这些突变体为测试病毒产物在自主细小病毒生物学中的作用提供了新工具。
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