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小鼠微小病毒cDNA的克隆及在鼠细胞中表达的单个病毒蛋白的初步分析。

Cloning of minute virus of mice cDNAs and preliminary analysis of individual viral proteins expressed in murine cells.

作者信息

Clemens K E, Cerutis D R, Burger L R, Yang C Q, Pintel D J

机构信息

Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri-Columbia 65212.

出版信息

J Virol. 1990 Aug;64(8):3967-73. doi: 10.1128/JVI.64.8.3967-3973.1990.

DOI:10.1128/JVI.64.8.3967-3973.1990
PMID:2164605
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249693/
Abstract

cDNAs corresponding to RNA from the autonomous parvovirus minute virus of mice were cloned into constitutive and inducible expression vectors. These clones generate viral NS2, VP1, and VP2 proteins individually. Initial examination of these clones by transient expression analysis and analysis of stably transformed murine cell lines inducibly expressing these constructs indicated that they will be useful tools for characterizing the function of individual minute virus of mice gene products.

摘要

将与来自小鼠自主细小病毒微小病毒的RNA对应的cDNA克隆到组成型和诱导型表达载体中。这些克隆分别产生病毒NS2、VP1和VP2蛋白。通过瞬时表达分析以及对可诱导表达这些构建体的稳定转化小鼠细胞系进行分析，对这些克隆进行的初步检测表明，它们将成为表征小鼠微小病毒单个基因产物功能的有用工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e93/249693/337c6a6213af/jvirol00063-0420-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e93/249693/fa9c973f552f/jvirol00063-0418-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e93/249693/724478204108/jvirol00063-0419-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e93/249693/337c6a6213af/jvirol00063-0420-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e93/249693/fa9c973f552f/jvirol00063-0418-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e93/249693/724478204108/jvirol00063-0419-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e93/249693/337c6a6213af/jvirol00063-0420-a.jpg

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本文引用的文献

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2
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3
Infection of eucaryotic cells by helper-independent recombinant adenoviruses: early region 1 is not obligatory for integration of viral DNA.
哺乳动物细胞周期调控细小病毒核衣壳组装。
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4
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