Damhorst Gregory L, Kooiman Jonathan M, Bashir Rashid
Annu Int Conf IEEE Eng Med Biol Soc. 2016 Aug;2016:5785-5788. doi: 10.1109/EMBC.2016.7592042.
Viral load quantification is a critical need for HIV management worldwide. However, the diagnostic technologies currently available are too limited by their size and expense to reach many remote and resource-limited populations. Toward the development of techniques which can be leveraged for point-of-care assays, we have investigated affinity capture of whole viruses using magnetic microparticles functionalized with antibodies or proteins targeting components of the HIV envelope. Results show the best performance from T-20, a small peptide employed in antiretroviral pharmacotherapy which targets an HIV envelope protein. This demonstration introduces an interesting alternative to antibodies for future affinity-capture applications in HIV diagnostics.
病毒载量定量是全球范围内艾滋病管理的一项关键需求。然而,目前可用的诊断技术在尺寸和成本方面受到极大限制,无法惠及许多偏远及资源有限地区的人群。为了开发可用于即时检测的技术,我们研究了使用针对HIV包膜成分的抗体或蛋白质功能化的磁性微粒对完整病毒进行亲和捕获。结果表明,抗逆转录病毒药物疗法中使用的一种靶向HIV包膜蛋白的小肽T - 20表现最佳。这一成果为未来HIV诊断中的亲和捕获应用提供了一种有趣的抗体替代方案。