血管活性肠肽结合位点在豚鼠生殖组织中的分布。

Distribution of vasoactive intestinal polypeptide binding sites in guinea pig genital tissues.

作者信息

Inyama C O, Wharton J, Davis C J, Jackson R H, Bloom S R, Polak J M

机构信息

Department of Histochemistry, Royal Postgraduate Medical School, London, U.K.

出版信息

Neurosci Lett. 1987 Oct 16;81(1-2):111-6. doi: 10.1016/0304-3940(87)90349-1.

DOI:10.1016/0304-3940(87)90349-1

PMID:2827063

Abstract

Vasoactive intestinal polypeptide (VIP) binding sites were localised in sections of guinea pig genital tissues using [125I]VIP and quantitative in vitro receptor autoradiography. A moderate density of binding was demonstrated in the epithelium of the prostate gland, seminal vesicles and in the testes. Moderate to high VIP binding also occurred in the epithelial lining of the oviduct, uterine glands, cervix and vagina. The binding sites appear to have a dissociation constant (Kd) of 0.36 nM and maximum receptor density (Bmax) of 60.5 x 10(-3) fmol/mm2 in sections of the uterine horn. The results indicate that VIP may have a role in the secretory functions of the guinea pig genital tract.

摘要

使用[125I]血管活性肠肽（VIP）和定量体外受体放射自显影技术，将VIP结合位点定位在豚鼠生殖组织切片中。在前列腺、精囊和睾丸的上皮中显示出中等密度的结合。在输卵管、子宫腺、子宫颈和阴道的上皮衬里中也出现中等至高的VIP结合。在子宫角切片中，结合位点的解离常数（Kd）为0.36 nM，最大受体密度（Bmax）为60.5×10（-3）fmol/mm2。结果表明，VIP可能在豚鼠生殖道的分泌功能中起作用。