Wolff Dietmar, Gerritzen Andreas
Clin Lab. 2017 Mar 1;63(3):603-606. doi: 10.7754/Clin.Lab.2016.160724.
A rapid molecular test for identifying cases of infectious diarrhea might be beneficial for patients.
We established a LightMix modular multiplex PCR assay for rapid detection of Campylobacter, Salmonella, Yersinia, and Shigella species from fecal samples.
Unlike Campylobacter sp., direct detection of Salmonella from feces by multiplex PCR was significantly less sensitive than culture. Only 11 out of 21 (52%) Salmonella culture positive specimens were positive for Salmonella in the direct multiplex PCR assay (Campylobacter: 52/53 samples [98%]). In contrast, an overnight selenite enrichment step prior to multiplex PCR increased the Salmonella assay sensitivity significantly: 18 out of 18 Salmonella culture positive samples were then also detected by PCR.
An overnight enrichment step is necessary for reliable PCR detection of Salmonella from fecal samples.
一种用于识别感染性腹泻病例的快速分子检测方法可能对患者有益。
我们建立了一种LightMix模块化多重PCR检测方法，用于从粪便样本中快速检测弯曲杆菌、沙门氏菌、耶尔森氏菌和志贺氏菌属。
与弯曲杆菌属不同，通过多重PCR直接从粪便中检测沙门氏菌的敏感性明显低于培养法。在21份沙门氏菌培养阳性标本中，直接多重PCR检测只有11份（52%）沙门氏菌呈阳性（弯曲杆菌：53份样本中有52份[98%]）。相比之下，在多重PCR之前进行过夜亚硒酸盐增菌步骤可显著提高沙门氏菌检测的敏感性：18份沙门氏菌培养阳性样本随后也通过PCR检测到。
要从粪便样本中可靠地通过PCR检测沙门氏菌，过夜增菌步骤是必要的。
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