B7-H3在乙型肝炎病毒感染进展过程中循环CD4+CD25高表达T细胞、循环CD14+单核细胞及血浆中的表达

The Expression of B7-H3 in Circulating CD4+CD25high T Cells, Circulating CD14+ Monocytes and Plasma during Hepatitis B Virus Infection Progression.

作者信息

Xu Jun-Chi, Li Ming, Ma Yuan, Shi Cui-Lin, Zhu Xiao-Yan, Song Hua-Feng, Chen Hui, Zhu Chuan-Wu, Xu Ping

出版信息

Clin Lab. 2017 Mar 1;63(3):617-623. doi: 10.7754/Clin.Lab.2016.160811.

DOI:10.7754/Clin.Lab.2016.160811

PMID:28271685

Abstract

BACKGROUND

B7-H3 is frequently upregulated in response to autoantigens and pathogens during host T cell immune responses. However, the role of B7-H3 which express in CD14+ monocytes and CD4+CD25high T cells had not been investigated.

METHODS

Cytometry and ELISA were used in this study.

RESULTS

The study showed that B7-H3 expression in CD14+ monocytes, CD4+CD25high T cells, and plasma was significantly increased in AHB, CHB, HBV-LC, and HBV-HCC group. In CHB group, the expression of B7-H3 was positively correlated with the ALT and AST levels.

CONCLUSIONS

B7-H3 expression in peripheral CD14+ monocytes, CD4+CD25high T cells, and plasma changed with HBV infection progression and had a significant correlation with liver function in CHB. B7-H3 expression could be utilized as a potential clinical indicator to determine the extent of liver injury.

摘要

背景

在宿主T细胞免疫反应过程中，B7-H3常因自身抗原和病原体而被上调表达。然而，B7-H3在CD14+单核细胞和CD4+CD25high T细胞中的表达作用尚未得到研究。

方法

本研究采用了流式细胞术和酶联免疫吸附测定法。

结果

研究表明，急性乙型肝炎（AHB）、慢性乙型肝炎（CHB）、乙型肝炎肝硬化（HBV-LC）和乙型肝炎肝癌（HBV-HCC）组中，CD14+单核细胞、CD4+CD25high T细胞及血浆中的B7-H3表达显著增加。在CHB组中，B7-H3的表达与谷丙转氨酶（ALT）和谷草转氨酶（AST）水平呈正相关。