含富血小板血浆的低分子量肝素-鱼精蛋白微/纳米颗粒提高全厚皮片存活率。

Improved Survival of Full-Thickness Skin Graft With Low-Molecular Weight Heparin-Protamine Micro/Nanoparticles Including Platelet-Rich Plasma.

作者信息

Takabayashi Yuki, Ishihara Masayuki, Kuwabara Masahiro, Takikawa Makoto, Nakamura Shingo, Hattori Hidemi, Kiyosawa Tomoharu

机构信息

From the *Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa; †Division of Biomedical Engineering, Research Institute, and ‡Department of Medical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan.

出版信息

Ann Plast Surg. 2017 May;78(5):562-568. doi: 10.1097/SAP.0000000000001051.

DOI:10.1097/SAP.0000000000001051

PMID:28272145

Abstract

BACKGROUND

Activated platelet-rich plasma secrets many growth factors (GFs), and low-molecular weight heparin-protamine micro/nanoparticles (LMWH-P M/NPs) significantly interact with, enhance, and stabilize the secreted GFs.

OBJECTIVE

The purpose of this study was to evaluate the effects of LMWH-P M/NPs and GFs (from platelet-rich plasma) on full-thickness skin graft (FTSG).

METHODS

A total of 96 inbred male rats were anesthetized and 4-cm full-thickness skin wound were created on dorsal skin of rats. LMWH-P M/NPs and GFs, LMWH-P M/NPs, GFs and saline (control) were then injected evenly into cutaneous muscles at the wound. The next day, the rats underwent FTSG. On the indicated days after FTSG, blood flow of FTSG site (wound bed and FTSG) was examined by 2-dimensional laser Doppler blood flowmeter. On 10 days, pictures of FTSG site were taken and FTSG survival rate was evaluated. Histologic analyses of skin samples were performed on 4, 7, and 10 days.

RESULTS

Treatment of full-thickness skin wound with LMWH-P M/NPs and GFs effectively promoted survival rate of FTSG and blood flow of FTSG site compared with those treated with GFs, LMWH-P M/NPs, and control. LMWH-P M/NPs and GFs also promoted new vessel formation at FTSG site.

CONCLUSIONS

The prior injection of LMWH-P M/NPs and GFs into wound bed increases FTSG survival rate, and promotes blood flow and angiogenesis at FTSG site.

摘要

背景

活化的富血小板血浆分泌多种生长因子（GFs），低分子量肝素 - 鱼精蛋白微/纳米颗粒（LMWH - P M/NPs）能与分泌的GFs显著相互作用、增强并稳定它们。

目的

本研究旨在评估LMWH - P M/NPs和GFs（来自富血小板血浆）对全层皮肤移植（FTSG）的影响。

方法

总共96只近交系雄性大鼠麻醉后，在其背部皮肤制作4厘米的全层皮肤伤口。然后将LMWH - P M/NPs和GFs、LMWH - P M/NPs、GFs和生理盐水（对照）均匀注射到伤口处的皮肤肌肉中。第二天，大鼠接受FTSG。在FTSG后的指定天数，用二维激光多普勒血流仪检查FTSG部位（伤口床和FTSG）的血流。在第10天，拍摄FTSG部位的照片并评估FTSG存活率。在第4、7和10天对皮肤样本进行组织学分析。