Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan.
Division of Biomedical Engineering, Research Institute, National Defense Medical College, Tokorozawa, Saitama, Japan.
J Surg Res. 2015 Jan;193(1):483-91. doi: 10.1016/j.jss.2014.08.011. Epub 2014 Aug 10.
Platelet-rich plasma (PRP) contains multiple growth factors, and fragmin-protamine micro-nanoparticles (F-P M-NPs) significantly enhance and stabilize growth factors. The purpose of this study was to evaluate the effects of PRP-containing F-P M-NPs (PRP&F-P M-NPs) on wound repair in split-thickness skin graft (STSG-) donor sites (DS).
A total of 56 inbred male rats were anesthetized and split-thickness skin graft donor site (STSG-DS) were created with a Padgett dermatome. PRP&F-P M-NPs, F-P M-NPs, PRP, and saline (control) were then intradermally injected evenly into the STSG-DSs. On 3, 4, 5, 7, and 10 d after creation of STSG-DS, skin sample sections were stained with hematoxylin and eosin to evaluate reepithelialization and angiogenesis.
Treatment of STSG-DS with PRP&F-P M-NPs effectively promoted epithelialization and new vessel formation compared with those treated with PRP, F-P M-NPs, and control (saline).
The intradermal injection of PRP&F-P M-NPs promotes epithelialization and angiogenesis in STSG-DS wounds.
富含血小板的血浆 (PRP) 中含有多种生长因子，而纤维蛋白原-鱼精蛋白微纳米颗粒 (F-P M-NPs) 可显著增强和稳定生长因子。本研究旨在评估含 PRP 的 F-P M-NPs (PRP&F-P M-NPs) 对供皮区 (DS) 全厚皮片移植 (STSG-) 创面修复的影响。
将 56 只近交雄性大鼠麻醉，用 Padgett 切皮机切取全厚皮片供区 (STSG-DS)。然后将 PRP&F-P M-NPs、F-P M-NPs、PRP 和生理盐水 (对照) 均匀地皮内注射到 STSG-DS 中。在 STSG-DS 形成后第 3、4、5、7 和 10 天，用苏木精和伊红染色皮肤标本切片，以评估上皮化和血管生成。
与 PRP、F-P M-NPs 和对照 (生理盐水) 处理的 STSG-DS 相比，PRP&F-P M-NPs 处理的 STSG-DS 能有效促进上皮化和新血管形成。
PRP&F-P M-NPs 皮内注射可促进 STSG-DS 创面的上皮化和血管生成。
