Lee Min Hyeock, Seo Hyun-Sun, Park Hyun Jin
Dept. of Biotechnology, College of Life Sciences and Biotechnology, Korea Univ., 5-Ka, Anam-Dong, SungBuk-Ku, Seoul, 136-701, Republic of Korea.
J Food Sci. 2017 Apr;82(4):922-932. doi: 10.1111/1750-3841.13675. Epub 2017 Mar 8.
An antimicrobial capsule releasing thyme oil was developed using modified halloysite nanotubes (HNTs). In order to increase the pore volume, HNTs were treated with 5.0 mol/L NaOH solution, which resulted in the encapsulation of more thyme oil molecules inside the HNTs. The morphology of the raw HNTs and NaOH-treated HNTs (N-HNTs) was characterized using transmission electron microscopy and nitrogen adsorption-desorption analysis. The loading capacity increased from 180.7 ± 12.7 to 256.4 ± 16.7 mg thyme oil/g HNT after the NaOH treatment. The aerial release characteristics of thyme oil from both the HNT capsules were investigated in a closed-package atmosphere system at 4, 25, and 40 °C. The antimicrobial activity of the capsule against Escherichia coli O157:H7 was determined using the vapor phase assay. Moreover, the antimicrobial effects of the capsule against E. coli O157:H7, total mesophilic aerobic bacteria (MAB), and molds and yeasts (MY) on the surfaces of cherry tomatoes were investigated at 4 and 25 °C for 5 d. When the cherry tomatoes were exposed to the thyme oil-loaded N-HNT capsule, the number of E. coli O157:H7, MAB, and MY significantly reduced during storage.
使用改性埃洛石纳米管（HNTs）制备了一种释放百里香油的抗菌胶囊。为了增加孔隙体积，用5.0 mol/L的氢氧化钠溶液处理HNTs，这使得更多的百里香油分子被包裹在HNTs内部。使用透射电子显微镜和氮吸附-脱附分析对原始HNTs和经氢氧化钠处理的HNTs（N-HNTs）的形态进行了表征。氢氧化钠处理后，负载量从180.7±12.7 mg百里香油/g HNT增加到256.4±16.7 mg百里香油/g HNT。在4、25和40°C的密闭包装气氛系统中研究了两种HNT胶囊中百里香油的空气释放特性。使用气相分析法测定了该胶囊对大肠杆菌O157:H7的抗菌活性。此外，研究了该胶囊在4和25°C下对樱桃番茄表面的大肠杆菌O157:H7、总嗜温需氧菌（MAB）以及霉菌和酵母菌（MY）的抗菌效果持续5天。当樱桃番茄暴露于负载百里香油的N-HNT胶囊时，储存期间大肠杆菌O157:H7、MAB和MY的数量显著减少。
