Institute of Fundamental Medicine and Biology , Kazan Federal University , Kreml uramı 18 , Kazan 420008 , Republic of Tatarstan , Russian Federation.
Dipartimento di Fisica e Chimica , Università degli Studi di Palermo , Viale delle Scienze, pad. 17 , Palermo 90128 , Italy.
ACS Appl Mater Interfaces. 2019 Jul 3;11(26):23050-23064. doi: 10.1021/acsami.9b07499. Epub 2019 Jun 21.
Alterations in the normal gastrointestinal microbial community caused by unhealthy diet, environmental factors, and antibiotic overuse may severely affect human health and well-being. Novel antimicrobial drug formulations targeting pathogenic microflora while not affecting or even supporting symbiotic microflora are urgently needed. Here we report fabrication of a novel antimicrobial nanocontainer based on halloysite nanotubes loaded with curcumin and protected with a dextrin outer layer (HNTs+Curc/DX) and its effective use to suppress the overgrowth of pathogenic bacteria in Caenorhabditis elegans nematodes. Nanocontainers have been obtained using vacuum-facilitated loading of hydrophobic curcumin into halloysite lumens. We have applied UV-vis and infrared spectroscopy, thermogravimetry and microscopy to characterize the HNTs+Curc/DX nanocontainers. In experiments in vitro we found that HNTs+Curc/DX effectively suppressed the growth of Serratia marcescens cells, whereas Escherichia coli bacteria were not affected. We applied HNTs+Curc/DX nanocontainers to alleviate the S. marcescens infection in C. elegans nematodes in vivo. The nematodes ingest HNTs+Curc/DX at 4-6 ng per worm, which results in improvement of the nematodes' fertility and life expectancy. Remarkably, treatment of S. marcescens-infected nematodes with HNTs+Curc/DX nanocontainers completely restored the longevity, demonstrating the enhanced bioavailability of hydrophobic curcumin. We believe that our results reported here open new avenues for fabrication of effective antimicrobial nanoformulations based on hydrophobic drugs and clay nanotubes.
不健康的饮食、环境因素和抗生素的过度使用导致正常胃肠道微生物群落发生改变，可能严重影响人类健康和幸福。迫切需要新型抗菌药物制剂来靶向致病微生物，而不影响甚至支持共生微生物。在这里，我们报告了一种基于负载姜黄素的多孔纳米管并用糊精外层保护的新型抗菌纳米容器的制备（HNTs+Curc/DX）及其在抑制秀丽隐杆线虫中致病性细菌过度生长方面的有效应用。纳米容器是通过真空辅助将疏水性姜黄素加载到多孔纳米管的腔室内而获得的。我们应用了紫外可见和红外光谱、热重分析和显微镜来对 HNTs+Curc/DX 纳米容器进行了表征。在体外实验中，我们发现 HNTs+Curc/DX 能有效抑制粘质沙雷氏菌细胞的生长，而大肠杆菌不受影响。我们将 HNTs+Curc/DX 纳米容器应用于体内缓解粘质沙雷氏菌感染秀丽隐杆线虫。线虫摄入每只线虫 4-6 纳克的 HNTs+Curc/DX，这导致线虫的繁殖力和预期寿命得到改善。值得注意的是，用 HNTs+Curc/DX 纳米容器处理粘质沙雷氏菌感染的线虫完全恢复了线虫的寿命，这表明疏水性姜黄素的生物利用度得到了提高。我们相信，我们在这里报告的结果为基于疏水性药物和粘土纳米管的有效抗菌纳米制剂的制备开辟了新的途径。
