Ramos-Oliveira Thayanne Monteiro, Silva Camila Vieira, Nunes Paula Mendes Acatauassu, Turssi Cecília Pedroso, Rechmann Peter, Freitas Patricia Moreira de
Universidade de São Paulo - USP, School of Dentistry, Department of Restorative Dentistry, São Paulo, SP, Brazil.
São Leopoldo Mandic Institute and Dental Research Center, Department of Restorative Dentistry, Campinas, SP, Brazil.
Braz Oral Res. 2017 Mar 6;31:e20. doi: 10.1590/1807-3107BOR-2017.vol31.0020.
This in situ study aimed to investigate the effect of a tin-containing fluoride solution in preventing enamel erosion. Also, its effects on the partly demineralized zone were assessed for the first time. Thirteen volunteers participated in this 2-phase study, wearing removable intra-oral appliances containing four sterilized bovine enamel slabs, for 8 days, where 2 treatment protocols were tested using samples in replicas (n = 13): CO - no treatment (negative control) and FL - AmF/NaF/SnCl2 solution (500 ppm F-, 800 ppm Sn2+, pH = 4.5). Samples were daily exposed to an erosive challenge (0.65% citric acid, pH 3.6, 4 min, 2x/day). In the 2nd phase, volunteers switched to the other treatment protocol. Samples were evaluated for surface loss using a profilometer (n = 13) and a cross-sectional nanohardness (CSNH) test (n = 13) was carried out in order to determine how deep the partly demineralized zone reaches below the erosive lesion. The data were statistically analyzed by two-way ANOVA. Erosive challenges lead to smaller enamel surface loss (p < 0.001) in the FL group when compared to group CO. Data from CSNH showed that there was no significant difference in demineralized enamel zone underneath erosion lesions between the groups. An amorphous layer could be observed on the surface of enamel treated with tin-containing solution alone. Under the experimental conditions of this in situ study, it can be concluded that AmF/NaF/SnCl2 solution prevents enamel surface loss but does not change the hardness of the partly demineralized zone near-surface enamel.
这项原位研究旨在调查含锡氟化物溶液在预防牙釉质侵蚀方面的效果。此外，还首次评估了其对部分脱矿区域的影响。13名志愿者参与了这项两阶段研究，佩戴含有四块消毒牛牙釉质板的可摘口腔矫治器8天，在复制品中使用样本测试了2种治疗方案（n = 13）：CO - 不治疗（阴性对照）和FL - 含氟亚锡/氟化钠/氯化亚锡溶液（500 ppm F-，800 ppm Sn2+，pH = 4.5）。样本每天接受侵蚀性刺激（0.65%柠檬酸，pH 3.6，4分钟，每天2次）。在第二阶段，志愿者切换到另一种治疗方案。使用轮廓仪评估样本的表面损失（n = 13），并进行横截面纳米硬度（CSNH）测试（n = 13），以确定部分脱矿区域在侵蚀性病变下方的深度。数据通过双向方差分析进行统计分析。与CO组相比，侵蚀性刺激导致FL组的牙釉质表面损失更小（p < 0.001）。CSNH数据显示，两组侵蚀性病变下方脱矿牙釉质区域没有显著差异。在仅用含锡溶液处理的牙釉质表面可观察到非晶层。在这项原位研究的实验条件下，可以得出结论，含氟亚锡/氟化钠/氯化亚锡溶液可防止牙釉质表面损失，但不会改变近表面牙釉质部分脱矿区域的硬度。
