de Oliveira Andrade Roberta, Kunitake Tiago, Koike Marcia Kiyomi, Machado Marcel C C, Souza Heraldo Possolo
Faculdade de Medicina da Universidade de São Paulo, Departamento de Emergências Clínicas, São Paulo/SP, Brazil.
Clinics (Sao Paulo). 2017 Feb 1;72(2):125-129. doi: 10.6061/clinics/2017(02)10.
: We aimed to assess the effects of diazoxide on the mortality, pancreatic injury, and inflammatory response in an experimental model of acute pancreatitis.
: Male Wistar rats (200-400 g) were divided randomly into two groups. Fifteen minutes before surgery, animals received physiological (0.9%) saline (3 mL/kg) (control group) or 45 mg/kg diazoxide (treatment group) via the intravenous route. Acute pancreatitis was induced by injection of 2.5% sodium taurocholate via the biliopancreatic duct. Mortality (n=38) was observed for 72 h and analyzed by the Mantel-Cox Log-rank test. To study pancreatic lesions and systemic inflammation, rats (10 from each group) were killed 3 h after acute pancreatitis induction; ascites volume was measured and blood as well as pancreases were collected. Pancreatic injury was assessed according to Schmidt's scale. Cytokine expression in plasma was evaluated by the multiplex method.
: Mortality at 72 h was 33% in the control group and 60% in the treatment group (p=0.07). Ascites volumes and plasma levels of cytokines between groups were similar. No difference was observed in edema or infiltration of inflammatory cells in pancreatic tissues from either group. However, necrosis of acinar cells was lower in the treatment group compared to the control group (3.5 vs. 3.75, p=0.015).
: Treatment with diazoxide can reduce necrosis of acinar cells in an experimental model of acute pancreatitis, but does not affect the inflammatory response or mortality after 72 h.
我们旨在评估二氮嗪对急性胰腺炎实验模型中死亡率、胰腺损伤及炎症反应的影响。
雄性Wistar大鼠（200 - 400克）随机分为两组。手术前15分钟，动物通过静脉途径接受生理盐水（0.9%）（3毫升/千克）（对照组）或45毫克/千克二氮嗪（治疗组）。通过经胆胰管注射2.5%牛磺胆酸钠诱导急性胰腺炎。观察72小时的死亡率（n = 38），并通过Mantel - Cox对数秩检验进行分析。为研究胰腺病变和全身炎症，在诱导急性胰腺炎3小时后处死大鼠（每组10只）；测量腹水量，并采集血液和胰腺。根据施密特量表评估胰腺损伤。通过多重方法评估血浆中细胞因子的表达。
对照组72小时死亡率为33%，治疗组为60%（p = 0.07）。两组间腹水量和细胞因子血浆水平相似。两组胰腺组织中炎症细胞的水肿或浸润均未观察到差异。然而，与对照组相比，治疗组腺泡细胞坏死程度较低（3.5对3.75，p = 0.015）。
在急性胰腺炎实验模型中，二氮嗪治疗可降低腺泡细胞坏死，但不影响72小时后的炎症反应或死亡率。
