拟南芥光合器官对 UV-B 的抗性因缺失光敏色素 B 和 A 而降低。
Resistance of Arabidopsis thaliana L. photosynthetic apparatus to UV-B is reduced by deficit of phytochromes B and A.
机构信息
Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region 142290, Russia; Institute for Biological Instrumentation, Russian Academy of Sciences, Institutskaya Street 7, Pushchino, Moscow Region 142290, Russia; Controlled Photobiosynthesis Laboratory, Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, Russia.
Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region 142290, Russia; Controlled Photobiosynthesis Laboratory, Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, Russia.
出版信息
J Photochem Photobiol B. 2017 Apr;169:41-46. doi: 10.1016/j.jphotobiol.2017.02.024. Epub 2017 Mar 1.
The photosynthetic responses of 25-day-old Arabidopsis phyA phyB double mutant (DM) compared with the wild type (WT) to UV-B radiation (1Wm, 30min) were investigated. UV-B irradiation led to reduction of photosystem 2 (PS-2) activity and the photosynthetic rate. In plants grown under both white and red light (λ - 660nm) the reduction was greater in DM plants compared to the WT. Without UV-B irradiation a decrease in PS-2 activity was observed in DM grown under RL only. It is assumed that the lower content of UV-absorbing pigments and carotenoids observed in DM may be one of the reasons of reduced PS-2 resistance to UV-B. Higher decrease in activities under UV in DM plants grown under RL compared to DM plants grown under white light is likely due to the lack of activity of cryptochromes in plants grown under red light. Rates of post-stress recovery of photosynthetic activity of DM compared with WT plants under white and red light of low intensity were studied. Almost complete recovery of the activity was found which was not observed under dark conditions and in the presence of a protein synthesis inhibitor, chloramphenicol. It is assumed that phytochrome system participates in stress-protective mechanisms of the photosynthetic apparatus to UV-radiation.
研究了 25 天大的拟南芥 phyA phyB 双突变体（DM）与野生型（WT）对 UV-B 辐射（1Wm，30min）的光合响应。UV-B 照射导致 PS-2 活性和光合速率降低。在白光和红光（λ - 660nm）下生长的植物中，与 WT 相比，DM 植物的减少更为明显。在没有 UV-B 照射的情况下，仅在 RL 下生长的 DM 中观察到 PS-2 活性下降。据认为，在 DM 中观察到的吸收紫外线的色素和类胡萝卜素含量较低可能是 PS-2 对 UV-B 抵抗力降低的原因之一。与在白光下生长的 DM 植物相比，在 RL 下生长的 DM 植物在 UV 下的活性下降幅度更高，这可能是由于在红光下生长的植物中隐色体缺乏活性。研究了在低强度白光和红光下 DM 与 WT 植物的光合活性在胁迫后的恢复率。在黑暗条件下和存在蛋白质合成抑制剂氯霉素的情况下，发现 DM 与 WT 植物的活性几乎完全恢复。据认为，光敏色素系统参与了光合作用器对 UV 辐射的应激保护机制。
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