Kreslavski Vladimir D, Khudyakova Alexandra Y, Kosobryukhov Anatoly A, Balakhnina Tamara I, Shirshikova Galina N, Alharby Hesham F, Allakhverdiev Suleyman I
Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, 142290 Pushchino, Russia.
Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Plants (Basel). 2023 Feb 15;12(4):867. doi: 10.3390/plants12040867.
The effects of heating (40 °C, 1 and 2 h) in dark and light conditions on the photosynthetic activity (photosynthesis rate and photosystem II activity), content of photosynthetic pigments, activity of antioxidant enzymes, content of thiobarbituric acid reactive substances (TBARs), and expression of a number of key genes of antioxidant enzymes and photosynthetic proteins were studied. It was shown that, in darkness, heating reduced CO gas exchange, photosystem II activity, and the content of photosynthetic pigments to a greater extent in the mutant than in the wild type (WT). The content of TBARs increased only in the mutant, which is apparently associated with a sharp increase in the total peroxidase activity in WT and its decrease in the mutant, which is consistent with a noticeable decrease in photosynthetic activity and the content of photosynthetic pigments in the mutant. No differences were indicated in all heated samples under light. It is assumed that the resistance of the photosynthetic apparatus to a short-term elevated temperature depends on the content of PHYB active form and is probably determined by the effect of phytochrome on the content of low-molecular weight antioxidants and the activity of antioxidant enzymes.
研究了在黑暗和光照条件下加热（40°C，1小时和2小时）对光合活性（光合速率和光系统II活性）、光合色素含量、抗氧化酶活性、硫代巴比妥酸反应性物质（TBARs）含量以及一些抗氧化酶和光合蛋白关键基因表达的影响。结果表明，在黑暗中，加热对突变体中CO气体交换、光系统II活性和光合色素含量的降低程度比对野生型（WT）更大。TBARs含量仅在突变体中增加，这显然与WT中总过氧化物酶活性的急剧增加及其在突变体中的降低有关，这与突变体中光合活性和光合色素含量的显著降低一致。在光照下，所有加热样品均未显示出差异。据推测，光合机构对短期高温的抗性取决于PHYB活性形式的含量，并且可能由光敏色素对低分子量抗氧化剂含量和抗氧化酶活性的影响所决定。
