Ranga Rao Suresh, Subbarayan Rajasekaran, Ajitkumar Supraja, Murugan Girija Dinesh
Head of Department, Department of Periodontics, Sri Ramachandra University , Chennai, Tamil Nadu, India .
Research Scholar, Centre for Regenerative Medicine and Stem Cell Research, Central Research Facility, Sri Ramachandra University , Chennai, Tamil Nadu, India .
J Clin Diagn Res. 2017 Jan;11(1):ZC49-ZC52. doi: 10.7860/JCDR/2017/22246.9183. Epub 2017 Jan 1.
Cyclosporin-A (CsA), an immunosuppressant, induces renal fibrosis and Renin Angiotensin System (RAS) is known to play a major role. CsA has the potential to increase the oxidative stress; specifically through the Advanced Oxidation Protein Products (AOPP) which could possibly stimulate fibrosis. A similar type of pathology occurs even in the gingiva known as CsA Induced Gingival Overgrowth (CIGO).
This study was undertaken to estimate the AOPP generation by Human Gingival Fibroblasts (HGF) under the influence of CsA and Angiotensin II (Ang II).
Six healthy gingival tissue samples were obtained during crown lengthening procedure and primary HGF were cultured using enzymatic digestion method. The ideal non-cytotoxic concentrations of CsA and Ang II were identified using cytotoxicity assay. Later, HGF were incubated with CsA and Ang II for 12 hours and AOPP assay was performed at zero and one hour interval.
There was a statistically significant increase in AOPP production in both the CsA and Ang II when compared to the control group with a p value<0.05.
CsA can induce oxidative stress and preventing/controlling it may be necessary to prevent untoward effect of the drug.
环孢素A（CsA）是一种免疫抑制剂，可诱导肾纤维化，已知肾素血管紧张素系统（RAS）在其中起主要作用。CsA有可能增加氧化应激；具体是通过晚期氧化蛋白产物（AOPP），其可能刺激纤维化。即使在牙龈中也会出现类似类型的病理情况，称为CsA诱导的牙龈过度生长（CIGO）。
本研究旨在评估在CsA和血管紧张素II（Ang II）影响下，人牙龈成纤维细胞（HGF）产生AOPP的情况。
在牙冠延长术期间获取6份健康牙龈组织样本，采用酶消化法培养原代HGF。使用细胞毒性试验确定CsA和Ang II的理想无细胞毒性浓度。随后，将HGF与CsA和Ang II孵育12小时，并在0小时和1小时间隔进行AOPP检测。
与对照组相比，CsA组和Ang II组的AOPP产量均有统计学显著增加，p值<0.05。
CsA可诱导氧化应激，预防/控制氧化应激可能对于预防该药物的不良影响是必要的。
