Supraja Ajitkumar, Dinesh Murugan Girija, Rajasekaran Subbarayan, Balaji Thodur Madapusi, Rao Suresh Ranga
Department of Periodontology and Implantology, Centre for Regenerative Medicine and Stem Cell Research, Sri Ramachandra University, Chennai, Tamil Nadu, India.
Centres for Indian Systems of Medicine Quality Assurance and Standardization, Sri Ramachandra University, Chennai, Tamil Nadu, India.
Dent Res J (Isfahan). 2016 Sep;13(5):405-412. doi: 10.4103/1735-3327.192276.
To evaluate the effect of Cyclosporin A (CsA) and angiotensin II (Ang II) on cytosolic calcium levels in cultured human gingival fibroblasts (HGFs).
Healthy gingival samples from six volunteers were obtained, and primary HGFs were cultured. Cell viability and proliferation assay were performed to identify the ideal concentrations of CsA and Ang II. Cytosolic calcium levels in cultured gingival fibroblasts treated with CsA and Ang II were studied using colorimetric assay, confocal and fluorescence imaging. Statistical analyses were done using SPSS software and GraphPad Prism.
Higher levels of cytosolic levels were evident in cells treated with CsA and Ang II when compared to control group and was statistically significant ( < 0.05) in both colorimetric assay and confocal imaging. Fluorescent images of the cultured HGFs revealed the same.
Thus calcium being a key player in major cellular functions, plays a major role in the pathogenesis of drug-induced gingival overgrowth.
评估环孢素A（CsA）和血管紧张素II（Ang II）对培养的人牙龈成纤维细胞（HGFs）胞浆钙水平的影响。
获取6名志愿者的健康牙龈样本，培养原代HGFs。进行细胞活力和增殖测定以确定CsA和Ang II的理想浓度。采用比色法、共聚焦和荧光成像研究用CsA和Ang II处理的培养牙龈成纤维细胞的胞浆钙水平。使用SPSS软件和GraphPad Prism进行统计分析。
与对照组相比，用CsA和Ang II处理的细胞中胞浆水平明显更高，在比色法和共聚焦成像中均具有统计学意义（<0.05）。培养的HGFs的荧光图像也显示了相同结果。
因此，钙作为主要细胞功能中的关键参与者，在药物性牙龈过度生长的发病机制中起主要作用。
