通过氢/氘交换（HDX）和傅里叶变换离子回旋共振质谱（FTICR MS）监测经二氢卟吩（DHPM）处理后卵清蛋白的功能特性和去折叠变化：经DHPM处理后卵清蛋白通过HDX和FTICR MS的功能与去折叠情况

Monitoring of the functional properties and unfolding change of Ovalbumin after DHPM treatment by HDX and FTICR MS: Functionality and unfolding of Oval after DHPM by HDX and FTICR MS.

作者信息

Liu Guang-Xian, Tu Zong-Cai, Wang Hui, Zhang Lu, Huang Tao, Ma Da

机构信息

Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China; Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China.

Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.

出版信息

Food Chem. 2017 Jul 15;227:413-421. doi: 10.1016/j.foodchem.2017.01.109. Epub 2017 Jan 25.

DOI:10.1016/j.foodchem.2017.01.109

Abstract

Dynamic high-pressure microfluidizaiton (DHPM) induced unfolding of proteins and enzymes could enhance the functional properties and hydrolytic ability of the molecules. In this study, the effect of DHPM on the surface activities of Ovalbumin (Oval), namely foaming and emulsifying properties, was investigated at 0, 40, 80, 120 and 160MPa. Improvement in the foaming and emulsifying properties was observed at 120 and 80MPa, respectively. Hydrogen/deuterium exchange (HDX) coupled with Fourier transform infrared spectroscopy-mass spectrometry (FTICR MS) was performed to investigate the exact unfolded region of Oval and analyze the mechanism promoting the foaming and emulsifying properties after 120 and 80MPa, respectively, compared with those at 0MPa. Prevalent unfolding of Oval after 80 and 120MPa treatment was revealed by HDX-MS, indicating a more flexible conformation induced by DHPM. However, lower deuterium incorporation was observed for the large six-stranded β-sheet. Therefore, the combined forces could enhance the flexibility and surface activities of Oval, which increased its foaming and emulsifying properties.

摘要

动态高压微射流（DHPM）诱导蛋白质和酶的展开可以增强分子的功能特性和水解能力。在本研究中，在0、40、80、120和160MPa下研究了DHPM对卵清蛋白（Oval）表面活性（即起泡和乳化性能）的影响。分别在120MPa和80MPa下观察到起泡和乳化性能的改善。进行了氢/氘交换（HDX）与傅里叶变换红外光谱-质谱联用（FTICR MS），以研究Oval的精确展开区域，并分别分析在120MPa和80MPa处理后与0MPa相比促进起泡和乳化性能的机制。HDX-MS揭示了80MPa和120MPa处理后Oval普遍展开，表明DHPM诱导了更灵活的构象。然而，对于大的六链β-折叠片观察到较低的氘掺入。因此，综合作用力可以增强Oval的柔韧性和表面活性，从而提高其起泡和乳化性能。