Rubin L J, Brown J E
Department of Ophthalmology, Washington University School of Medicine, St. Louis, Missouri 63110.
Am J Physiol. 1988 Jan;254(1 Pt 1):C27-36. doi: 10.1152/ajpcell.1988.254.1.C27.
Firefly luciferin-luciferase was microinjected into single, intact photoreceptor cells of Limulus ventral eyes. In addition, total ATP content of whole end organs was measured. An attempt was made to correlate intracellular ATP levels with either the frequency of discrete events or responsiveness to light. Luciferin-luciferase luminescence emitted from a single photoreceptor cell increased after an injection of ATP or P[NH]P and decreased after injection of apyrase. We conclude that the luciferin-luciferase luminescence is monitoring increases as well as decreases in intracellular ATP concentration (ATPi). The ATPi was between 10(-3) and 10(-4) M. Bathing ventral eyes in vanadate or fluoride increased ATP content of whole end organs, increased ATPi in single photoreceptors, and increased the frequency of discrete events. However, apyrase decreased ATPi but did not affect the frequency of discrete events or responsiveness to light until ATPi was dramatically reduced. We conclude that changes in ATP levels are not strictly correlated with changes in responsiveness to light or to changes in the frequency of discrete events.
将萤火虫荧光素 - 荧光素酶显微注射到美洲鲎腹眼的单个完整光感受器细胞中。此外，还测量了整个终器的总ATP含量。尝试将细胞内ATP水平与离散事件的频率或对光的反应性相关联。注射ATP或P[NH]P后，单个光感受器细胞发出的荧光素 - 荧光素酶发光增加，而注射腺苷三磷酸双磷酸酶后发光减少。我们得出结论，荧光素 - 荧光素酶发光监测细胞内ATP浓度（ATPi）的增加和减少。ATPi在10^(-3)和10^(-4) M之间。用钒酸盐或氟化物浸泡腹眼会增加整个终器的ATP含量，增加单个光感受器中的ATPi，并增加离散事件的频率。然而，腺苷三磷酸双磷酸酶降低了ATPi，但在ATPi显著降低之前，并不影响离散事件的频率或对光的反应性。我们得出结论，ATP水平的变化与对光的反应性变化或离散事件频率的变化并不严格相关。
