Minami R, Yokota S, Teplitz R L
Acta Cytol. 1978 Nov-Dec;22(6):584-8.
Data were obtained concerning the usefulness of discontinuous Ficoll and Isopaque-Ficoll gradients in separating malignant from non-malignant cells from a variety of sources. Most of the material was from effusions, but there is no theoretical restriction of the methods to effusions. Animal and human material were studied, utilizing non-malignant as well as malignant material, the former acting as controls. In the discontinuous Ficoll gradient, concentration of malignant cells at a specific interface was mainly a function of cell density. Thus, while most malignant cells banded in the last two fractions, adenocarcinomas, especially those high in mucus (which decreases total cell density) banded higher in the gradient. The Isopaque-Ficoll technique also proved useful in concentrating malignant cells. The advantage of the discontinuous Ficoll gradient was its ability to separate cells from small specimens (1-10 X 10(6) cells). Contrarily, the Isopaque-Ficoll gradient was preferable for processing specimens of larger volumes.
获得了关于间断性聚蔗糖和异泛影葡胺 - 聚蔗糖梯度在从多种来源的标本中分离恶性细胞与非恶性细胞方面的实用性的数据。大部分标本来自积液，但从理论上讲，这些方法并不局限于积液。研究了动物和人类标本，同时使用了非恶性和恶性标本，前者作为对照。在间断性聚蔗糖梯度中，特定界面处恶性细胞的浓度主要取决于细胞密度。因此，虽然大多数恶性细胞聚集在最后两个组分中，但腺癌，尤其是那些黏液含量高（这会降低总细胞密度）的腺癌，在梯度中聚集得更高。异泛影葡胺 - 聚蔗糖技术在浓缩恶性细胞方面也被证明是有用的。间断性聚蔗糖梯度的优点是能够从小标本（1 - 10×10⁶个细胞）中分离细胞。相反，异泛影葡胺 - 聚蔗糖梯度更适合处理较大体积的标本。
