Christensen L S, Soerensen K J, Lei J C
State Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark.
Arch Virol. 1987;97(3-4):215-24. doi: 10.1007/BF01314422.
Purified DNA from 42 isolates of Suid herpesvirus 1 (SHV-1) collected during 1985 from clinical outbreaks of Aujezsky's disease on Danish farms was compared by restriction fragment pattern (RFP) analysis. The BamHI generated RFPs were found to be distinguishable, thus confirming RFP analysis as a potential epidemiological tool. However, because of variations within labile genome regions of epidemiologically closely related isolates, it was concluded that comparisons of RFPs should be interpreted with great care and only on the basis of a comprehensive reference material. With this proviso, the changes in RFPs occurring during the spread of the infection might be useful markers in the evaluation of epidemiological relationships. A simple procedure for obtaining satisfactory amounts of purified SHV-1 DNA is described.
对1985年从丹麦农场发生的奥耶斯基氏病临床疫情中收集的42株猪疱疹病毒1型（SHV-1）的纯化DNA，通过限制性片段图谱（RFP）分析进行了比较。发现BamHI产生的RFP是可区分的，从而证实RFP分析是一种潜在的流行病学工具。然而，由于在流行病学上密切相关的分离株的不稳定基因组区域内存在变异，得出的结论是，RFP的比较应非常谨慎地进行，并且只能基于全面的参考材料。在这一前提下，感染传播过程中RFP的变化可能是评估流行病学关系的有用标记。描述了一种获得足量纯化SHV-1 DNA的简单方法。
