Avila-George K, Ramos-Olivares K, Vasquez-Munoz K, Villanueva-Morales V, Reyes-Farias M, Quintero P, Garcia L, Garcia-Diaz D F
a Department of Nutrition , Faculty of Medicine, University of Chile , Santiago , Chile.
b Department of Gastroenterology , Faculty of Medicine, Pontifical Catholic University of Chile , Santiago , Chile , and.
Arch Physiol Biochem. 2017 Jul;123(3):175-181. doi: 10.1080/13813455.2017.1285318. Epub 2017 Feb 14.
Expansion of white adipose tissue induce insufficient vascularization, driving hypoxia and low-grade inflammation. Resident preadipocytes are thought to be involved. We evaluated the effects of hypoxia over preadipocytes and adipocytes, to determine which cellular type impacts the most over macrophages activation. 3T3-L1 cells were either differentiated, or maintained undifferentiated. Each group was subjected to the presence or absence of chemical hypoxia (200 μM CoCl) for 24 h. Conditioned media were used as treatment for murine RAW264.7 macrophages for 24 h. Gene expression of HIF-1α and TNF-α, and the release of several markers were assessed. It was observed that culture media from hypoxic preadipocytes induced greater expression of inflammatory markers and NO release than culture media from hypoxic adipocytes, by macrophages. Gene expression correlated closer with inflammatory markers release specially on macrophages treated with conditioned media from preadipocytes. Hence, the present work highlights the importance of preadipocytes on inflammatory conditions in vitro.
白色脂肪组织的扩张会导致血管生成不足，引发缺氧和低度炎症。常驻前脂肪细胞被认为与此有关。我们评估了缺氧对前脂肪细胞和脂肪细胞的影响，以确定哪种细胞类型对巨噬细胞激活的影响最大。将3T3-L1细胞进行分化或维持未分化状态。每组细胞分别在有或没有化学性缺氧（200μM氯化钴）的条件下培养24小时。用条件培养基处理小鼠RAW264.7巨噬细胞24小时。评估缺氧诱导因子-1α（HIF-1α）和肿瘤坏死因子-α（TNF-α）的基因表达以及几种标志物的释放情况。结果发现，与来自缺氧脂肪细胞的培养基相比，巨噬细胞对来自缺氧前脂肪细胞的培养基诱导的炎症标志物表达和一氧化氮（NO）释放更为明显。基因表达与炎症标志物的释放密切相关，特别是在用前脂肪细胞条件培养基处理的巨噬细胞中。因此，本研究突出了前脂肪细胞在体外炎症条件下的重要性。
