Neary J T, Norenberg L O, Gutierrez M P, Norenberg M D
Laboratory of Neuropathology, Veterans Administration Medical Center, Miami, FL.
Brain Res. 1987 Dec 22;437(1):161-4. doi: 10.1016/0006-8993(87)91538-1.
Treatment of primary astrocyte cultures with ammonium chloride for one day prior to phosphoprotein labeling resulted in a reduction in phosphate incorporation in a 66-kDa protein. Increasing ammonium chloride concentrations (2, 5, and 10 mM) led to greater reductions in phosphate incorporation in this band. The specificity of the effect was indicated by the lack of change in phosphate incorporation in 7 other protein bands. These results indicate that protein phosphorylation can be affected by pathophysiological concentrations of ammonia and suggest that altered protein phosphorylation may be related to the pathogenesis of disorders such as hepatic encephalopathy and Reye's syndrome where ammonia has been implicated as an important etiological factor.
在进行磷蛋白标记前一天，用氯化铵处理原代星形胶质细胞培养物，导致一种66 kDa蛋白的磷酸盐掺入减少。增加氯化铵浓度（2、5和10 mM）会使该条带中的磷酸盐掺入进一步减少。其他7条蛋白条带的磷酸盐掺入没有变化，表明了这种效应的特异性。这些结果表明，蛋白质磷酸化会受到病理生理浓度氨的影响，并提示蛋白质磷酸化改变可能与诸如肝性脑病和瑞氏综合征等疾病的发病机制有关，在这些疾病中氨被认为是一个重要的病因。
