Kramer S M, Aggarwal B B, Eessalu T E, McCabe S M, Ferraiolo B L, Figari I S, Palladino M A
Department of Assay Development, Genentech, Inc., South San Francisco, California 94080.
Cancer Res. 1988 Feb 15;48(4):920-5.
The species preference of human and murine tumor necrosis factor-alpha (TNF-alpha) was evaluated in human and murine systems for cytotoxic/cytostatic effects and receptor binding in vitro and murine systems for toxicity and antitumor activity in vivo. The in vitro cytotoxic/cytostatic effects of both species TNF-alpha on human and murine cell lines as well as the receptor binding studies using 125I-labeled recombinant human TNF-alpha demonstrated homologous species preferences. Species preference of TNF-alpha was also apparent in toxicity studies with BALB/c nu/nu and CB6F1 mice, and antitumor responses of CB6F1 mice to s.c. Meth A sarcoma implants. Moreover the growth of Meth A sarcoma implanted i.p. was not inhibited by either human or murine TNF-alpha. These results are discussed in view of the potential for underestimation of the biological potency of TNF-alpha from heterologous sources.
在人和小鼠系统中评估了人源和鼠源肿瘤坏死因子-α（TNF-α）的物种偏好，包括体外细胞毒性/细胞抑制作用和受体结合，以及在小鼠系统中的体内毒性和抗肿瘤活性。两种物种的TNF-α对人和小鼠细胞系的体外细胞毒性/细胞抑制作用，以及使用125I标记的重组人TNF-α进行的受体结合研究均显示出同源物种偏好。TNF-α的物种偏好在对BALB/c裸鼠和CB6F1小鼠的毒性研究以及CB6F1小鼠对皮下接种Meth A肉瘤植入物的抗肿瘤反应中也很明显。此外，腹腔内植入的Meth A肉瘤的生长不受人源或鼠源TNF-α的抑制。鉴于可能低估来自异源的TNF-α的生物学效力，对这些结果进行了讨论。
