Dallegri F, Frumento G, Ballestrero A, Goretti R, Patrone F
First Medical Clinic, University of Genova Medical School, Italy.
Clin Exp Immunol. 1987 Nov;70(2):479-83.
Neutrophil-mediated antibody-dependent cellular cytotoxicity (ADCC) against Raji target cells and neutrophil degranulation during the ADCC process were evaluated in the presence and in the absence of different agents able to interfere with the neutrophil release of granule components (anion channel blockers, colchicine, isoproterenol, dimethylxanthine, cAMP). When used at concentrations incapable of preventing the target cell recognition by neutrophils, the majority of these agents inhibited both the ADCC and the release of myeloperoxidase (MPO, primary granule marker) and lysozyme (LZM, primary and secondary granule marker). The inhibition of the ADCC correlated strictly with the inhibition of the MPO release. Thus, the results are consistent with the hypothesis that neutrophil primary granules play a major role in the cytolytic process.
在存在和不存在能够干扰中性粒细胞颗粒成分释放的不同试剂（阴离子通道阻滞剂、秋水仙碱、异丙肾上腺素、二甲基黄嘌呤、环磷酸腺苷）的情况下，评估了中性粒细胞介导的针对Raji靶细胞的抗体依赖性细胞毒性（ADCC）以及ADCC过程中的中性粒细胞脱颗粒情况。当以无法阻止中性粒细胞识别靶细胞的浓度使用时，这些试剂中的大多数均抑制了ADCC以及髓过氧化物酶（MPO，初级颗粒标志物）和溶菌酶（LZM，初级和次级颗粒标志物）的释放。ADCC的抑制与MPO释放的抑制密切相关。因此，这些结果与中性粒细胞初级颗粒在细胞溶解过程中起主要作用的假设一致。
