Dai Xin, Shi Jia, Han Mei, Wang Ai Qin, Wei Wan Hong, Yang Sheng Mei
College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China.
College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, PR China.
Gen Comp Endocrinol. 2017 May 15;246:1-8. doi: 10.1016/j.ygcen.2017.03.003. Epub 2017 Mar 6.
Plant secondary metabolite 6-methoxybenzoxazolinone (6-MBOA) has been suggested to stimulate animal reproduction. 6-MBOA is detected in Leymus chinensis, a main diet of Brandt's vole (Lasiopodomys brandtii). We have previously reported a stimulatory effect of 6-MBOA on reproduction of male Brandt's voles under a short-day photoperiod. The goal of this study was to investigate the effect of 6-MBOA on reproductive physiology of male Brandt's voles under a long-day photoperiod and examine if 6-MBOA under this photoperiodic regime altered the reproductive status of male Brandt's voles differently than the short-day photoperiod. Under the long-day photoperiod, a high dose of 6-MBOA decreased KiSS-1 mRNA in the arcuate nucleus (ARC), and we also saw a decrease in circulating levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone (T). Steroidogenic acute regulatory protein (StAR) and cytochrome P450 (CYP11a1) in the testes, and relative testis weight also decreased with 6-MBOA administration. Compared to the short-day photoperiod, animals under the long-day photoperiod exhibited increased body weight as well as all other reproductive parameters. Our results showed that 6-MBOA inhibited the reproduction of male Brandt's vole under a long-day photoperiod, a stark contrast from its stimulatory effects under a short-day photoperiod. The paradoxical effects of 6-MBOA suggest it may act as a partial agonist of melatonin. These results provide insight into the complex interactions between environmental factors such as photoperiod and diet in the control of Brandt's vole reproduction.
植物次生代谢产物6-甲氧基苯并恶唑啉酮（6-MBOA）被认为可以刺激动物繁殖。在布氏田鼠（Lasiopodomys brandtii）的主要食物羊草中可检测到6-MBOA。我们之前报道过，在短日照光周期下，6-MBOA对雄性布氏田鼠的繁殖有刺激作用。本研究的目的是调查在长日照光周期下6-MBOA对雄性布氏田鼠生殖生理学的影响，并检验在这种光周期条件下6-MBOA是否会以与短日照光周期不同的方式改变雄性布氏田鼠的生殖状态。在长日照光周期下，高剂量的6-MBOA降低了弓状核（ARC）中KiSS-1 mRNA的水平，我们还观察到促黄体生成素（LH）、促卵泡生成素（FSH）和睾酮（T）的循环水平有所下降。随着6-MBOA的给药，睾丸中的类固醇生成急性调节蛋白（StAR）和细胞色素P450（CYP11a1）以及相对睾丸重量也有所下降。与短日照光周期相比，长日照光周期下的动物体重以及所有其他生殖参数均有所增加。我们的结果表明，在长日照光周期下，6-MBOA抑制了雄性布氏田鼠的繁殖，这与它在短日照光周期下的刺激作用形成了鲜明对比。6-MBOA的这种矛盾效应表明它可能作为褪黑素的部分激动剂发挥作用。这些结果为光周期和饮食等环境因素在控制布氏田鼠繁殖过程中的复杂相互作用提供了见解。
