Cheng B, Horst I A, Kowal J
Department of Medicine, Case Western Reserve University, Cleveland, Ohio.
Endocr Res. 1987;13(3):243-50. doi: 10.1080/07435808709035456.
Following 15-30 min exposure to monensin, adrenocorticotropic hormone (ACTH)-stimulated steroidogenesis in cultured adrenal cells is inhibited by 37-48%. Electron microscopic studies reveal that, in monensin-treated cells, the Golgi complexes are disrupted into large vacuolar structures with loss of its organized structure indicating that the action of monensin on the organelles is comparably rapid. The inhibition is fully reversed after removal of the monensin-containing medium and exposure to fresh growth medium for a subsequent 4-24 h prior to stimulation. Concomitant with the restoration of full steroidogenic activity, the disrupted organelles are extensively reorganized in the cells after exposure to fresh growth medium for 4-24 h. These findings, which demonstrate, for the first time, a correlation between the morphology of the Golgi complex and steroidogenic activity, strengthen the possibility that the organelle may be involved in the regulation of steroidogenesis.
在接触莫能菌素15 - 30分钟后，培养的肾上腺细胞中促肾上腺皮质激素（ACTH）刺激的类固醇生成受到37 - 48%的抑制。电子显微镜研究显示，在经莫能菌素处理的细胞中，高尔基体复合物被破坏成大的液泡状结构，其有组织的结构丧失，这表明莫能菌素对细胞器的作用相当迅速。在去除含莫能菌素的培养基并在刺激前将细胞暴露于新鲜生长培养基中4 - 24小时后，抑制作用完全逆转。在暴露于新鲜生长培养基4 - 24小时后，细胞内被破坏的细胞器广泛重组，同时类固醇生成活性完全恢复。这些首次证明高尔基体复合物形态与类固醇生成活性之间存在关联的发现，增强了该细胞器可能参与类固醇生成调节的可能性。
