Kim Eunbi, Na Sunghun, An Borim, Yang Se-Ran, Kim Woo Jin, Ha Kwon-Soo, Han Eun-Taek, Park Won Sun, Lee Chang-Min, Lee Ji Yoon, Lee Seung-Joon, Hong Seok-Ho
Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
Department of Obstetrics & Gynecology, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
Korean J Physiol Pharmacol. 2017 Mar;21(2):161-168. doi: 10.4196/kjpp.2017.21.2.161. Epub 2017 Feb 21.
Understanding the crosstalk mechanisms between perivascular cells (PVCs) and cancer cells might be beneficial in preventing cancer development and metastasis. In this study, we investigated the paracrine influence of PVCs derived from human umbilical cords on the proliferation of lung adenocarcinoma epithelial cells (A549) and erythroleukemia cells (TF-1α and K562) using Transwell® co-culture systems. PVCs promoted the proliferation of A549 cells without inducing morphological changes, but had no effect on the proliferation of TF-1α and K562 cells. To identify the factors secreted from PVCs, conditioned media harvested from PVC cultures were analyzed by antibody arrays. We identified a set of cytokines, including persephin (PSPN), a neurotrophic factor, and a key regulator of oral squamous cell carcinoma progression. Supplementation with PSPN significantly increased the proliferation of A549 cells. These results suggested that PVCs produced a differential effect on the proliferation of cancer cells in a cell-type dependent manner. Further, secretome analyses of PVCs and the elucidation of the molecular mechanisms could facilitate the discovery of therapeutic target(s) for lung cancer.
了解血管周围细胞（PVCs）与癌细胞之间的串扰机制可能有助于预防癌症的发生和转移。在本研究中，我们使用Transwell®共培养系统研究了源自人脐带的PVCs对肺腺癌上皮细胞（A549）以及红白血病细胞（TF-1α和K562）增殖的旁分泌影响。PVCs促进了A549细胞的增殖，且未诱导形态变化，但对TF-1α和K562细胞的增殖没有影响。为了鉴定PVCs分泌的因子，通过抗体阵列分析了从PVC培养物中收集的条件培养基。我们鉴定出一组细胞因子，包括神经营养因子persephin（PSPN），它是口腔鳞状细胞癌进展的关键调节因子。补充PSPN显著增加了A549细胞的增殖。这些结果表明，PVCs以细胞类型依赖的方式对癌细胞的增殖产生了不同的影响。此外，对PVCs的分泌蛋白组分析以及分子机制的阐明可能有助于发现肺癌的治疗靶点。
