Sabetian Soudabeh, Shamsir Mohd Shahir
Department of Biological and Health Sciences, Faculty of Bioscience & Medical Engineering, Universiti Teknologi Malaysia, 81310, Johor, Malaysia.
J Membr Biol. 2017 Apr;250(2):133-144. doi: 10.1007/s00232-017-9954-1. Epub 2017 Mar 9.
Complete elucidation of fertilization process at molecular level is one of the unresolved challenges in sexual reproduction studies, and understanding the molecular mechanism is crucial in overcoming difficulties in infertility and unsuccessful in vitro fertilization. Sperm-oocyte interaction is one of the most remarkable events in fertilization process, and deficiency in protein-protein interactions which mediate this interaction is a major cause of unexplained infertility. Due to detection of how the various defects of sperm-oocyte interaction can affect fertilization failure, different experimental methods have been applied. This review summarizes the current understanding of sperm-egg interaction mechanism during fertilization and also accumulates the different types of sperm-egg interaction abnormalities and their association with infertility. Several detection approaches regarding sperm-egg protein interactions and the associated defects are reviewed in this paper.
在分子水平上完整阐明受精过程是有性生殖研究中尚未解决的挑战之一，而理解分子机制对于克服不孕症和体外受精失败的难题至关重要。精子与卵母细胞的相互作用是受精过程中最显著的事件之一，介导这种相互作用的蛋白质 - 蛋白质相互作用缺陷是不明原因不孕症的主要原因。由于检测到精子与卵母细胞相互作用的各种缺陷如何影响受精失败，人们应用了不同的实验方法。本文综述了目前对受精过程中精卵相互作用机制的理解，也总结了不同类型的精卵相互作用异常及其与不孕症的关联。本文还综述了几种关于精卵蛋白质相互作用及其相关缺陷的检测方法。
