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海鳗中钠钾ATP酶对盐度变化的响应及其与FXYD11表达的相关性

Na/K-ATPase response to salinity change and its correlation with FXYD11 expression in Anguilla marmorata.

作者信息

Liang Fenfei, Li Li, Zhang Guosong, Yin Shaowu, Wang Xiaolu, Li Peng, Jia Yihe, Wang Yayuan, Wang Li, Wang Xiaojun

机构信息

Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.

Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, 222005, China.

出版信息

J Comp Physiol B. 2017 Oct;187(7):973-984. doi: 10.1007/s00360-017-1059-8. Epub 2017 Mar 9.

DOI:10.1007/s00360-017-1059-8
PMID:28280923
Abstract

The Na/K-ATPase (NKA) is a primary electrogenic protein that promotes ion transport in teleosts. FXYD11 is a putative regulatory subunit of the NKA pump. The regulation of Na /K -ATPase and FXYD11 is of critical importance for osmotic homeostasis. To investigate the changes of the two genes under different salinity environments, we first identified NKA (AmNKAα1) and FXYD11 (AmFXYD11) in Anguilla marmorata, and then evaluated the mRNA levels of NKA and FXYD11 as well as the activity of NKA in the gill and kidney at different timepoints (0, 1, 3, 6, 12, 24, 48, 72, 96, and 360 h) under three salinity conditions-0‰ (fresh water: FW), 10‰ (brackish water: BW), and 25‰ (seawater: SW). In the gill, the mRNA levels of AmNKAα1 and AmFXYD11 and the enzyme activity of AmNKAα1 were higher in BW and SW than in FW; the protein abundance was positively correlated with the specific activity of NKA in BW/SW. However, in the kidney, the mRNA level of AmNKAα1 in the BW group was higher than that in the FW group. In addition, AmFXYD mRNA levels in both BW and SW groups were significantly lower than that in the FW control group. These results suggested that AmFXYD11 was tissue specific in response to different salinity environment. Our results clearly demonstrated the important roles of AmNKAα1 and AmFXYD11 in osmotic homeostasis of juvenile A. marmorata under saline environment.

摘要

钠钾-ATP酶（NKA）是一种主要的生电蛋白，可促进硬骨鱼中的离子运输。FXYD11是NKA泵的一种假定调节亚基。钠钾-ATP酶和FXYD11的调节对于渗透稳态至关重要。为了研究这两个基因在不同盐度环境下的变化，我们首先在斑纹鳗鲡中鉴定出NKA（AmNKAα1）和FXYD11（AmFXYD11），然后评估了在三种盐度条件下（0‰（淡水：FW）、10‰（咸水：BW）和25‰（海水：SW））不同时间点（0、1、3、6、12、24、48、72、96和360小时）鳃和肾脏中NKA和FXYD11的mRNA水平以及NKA的活性。在鳃中，BW和SW组中AmNKAα1和AmFXYD11的mRNA水平以及AmNKAα1的酶活性均高于FW组；蛋白质丰度与BW/SW组中NKA的比活性呈正相关。然而，在肾脏中，BW组中AmNKAα1的mRNA水平高于FW组。此外，BW和SW组中AmFXYD的mRNA水平均显著低于FW对照组。这些结果表明，AmFXYD11在响应不同盐度环境时具有组织特异性。我们的结果清楚地证明了AmNKAα1和AmFXYD11在咸水环境下幼年斑纹鳗鲡渗透稳态中的重要作用。

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