Owen A, Malaisse W J
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Diabete Metab. 1987 Sep-Oct;13(5):514-9.
Recent experimental data suggest that a rise in cyclic AMP content of pancreatic islet cells may have little effect upon cytosolic Ca2+ activity. Hence, a mathematical model for stimulus-secretion coupling was designed in which cyclic AMP fails to affect Ca2+ movements in the islet cells, but augments the responsiveness to cytosolic Ca2+ of the effector system for insulin release. Provided that the latter effect of cyclic AMP becomes operative only when the nucleotide concentration exceeds a critical threshold value, the mathematical model was found suitable to predict the dynamics of both insulin release and 45Ca fluxes in islets deprived of or exposed to D-glucose, Ca2+ and a phosphodiesterase inhibitor.
最近的实验数据表明，胰岛细胞中环状AMP含量的升高可能对胞质Ca2+活性影响甚微。因此，设计了一种刺激-分泌偶联的数学模型，其中环状AMP不会影响胰岛细胞中的Ca2+运动，但会增强胰岛素释放效应系统对胞质Ca2+的反应性。假设环状AMP的后一种效应仅在核苷酸浓度超过临界阈值时才起作用，则发现该数学模型适合预测在缺乏或暴露于D-葡萄糖、Ca2+和磷酸二酯酶抑制剂的胰岛中胰岛素释放和45Ca通量的动态变化。
