儿茶酚胺对离体鲶鱼肝细胞中环磷酸腺苷水平的影响。

Catecholamine effect on cyclic adenosine 3':5'-monophosphate level in isolated catfish hepatocytes.

作者信息

Brighenti L, Puviani A C, Gavioli M E, Fabbri E, Ottolenghi C

机构信息

Institute of General Physiology, University of Ferrara, Italy.

出版信息

Gen Comp Endocrinol. 1987 Nov;68(2):216-23. doi: 10.1016/0016-6480(87)90032-3.

DOI:10.1016/0016-6480(87)90032-3

PMID:2828149

Abstract

The effect of catecholamines (epinephrine, norepinephrine, isoproterenol, and phenylephrine) on cyclic adenosine 3':5'-monophosphate (cAMP) level in isolated catfish (Ictalurus melas) liver cells was studied in the presence or absence of alpha (phentolamine) and beta (propranolol)-receptor antagonists. All catecholamines increased the hepatocyte cAMP level: the rank of their potency was epinephrine = isoproterenol greater than norepinephrine greater than phenylephrine. Propranolol completely blocked the catecholamine effect; phentolamine was ineffective. Results confirm previous findings (L. Brighenti, A. C. Puviani, M. E. Gavioli, and C. Ottolenghi, 1987, Gen. Comp. Endocrinol. 66, 306-313) that epinephrine and norepinephrine act via beta-receptor activation. However, the comparison of the effects of isoproterenol and phenylephrine on cAMP with those on phosphorylase alpha and on glycogen breakdown suggests that a more complex mechanism is possibly involved in the catecholamine effect on catfish glycogenolysis.

摘要

在存在或不存在α（酚妥拉明）和β（普萘洛尔）受体拮抗剂的情况下，研究了儿茶酚胺（肾上腺素、去甲肾上腺素、异丙肾上腺素和去氧肾上腺素）对离体鲶鱼（黑叉尾鮰）肝细胞中环磷酸腺苷（cAMP）水平的影响。所有儿茶酚胺均增加了肝细胞cAMP水平：其效力排序为肾上腺素 = 异丙肾上腺素＞去甲肾上腺素＞去氧肾上腺素。普萘洛尔完全阻断了儿茶酚胺的作用；酚妥拉明无效。结果证实了先前的研究结果（L. 布里根蒂、A. C. 普维亚尼、M. E. 加廖利和C. 奥托伦吉，1987年，《普通与比较内分泌学》66卷，第306 - 313页），即肾上腺素和去甲肾上腺素通过β受体激活发挥作用。然而，将异丙肾上腺素和去氧肾上腺素对cAMP的作用与它们对磷酸化酶α和糖原分解的作用进行比较表明，儿茶酚胺对鲶鱼糖原分解的作用可能涉及更复杂的机制。