Pasleau F, Leung F, Kopchick J J
Department of Animal Drug Discovery, Merck Institute for Therapeutic Research, Rahway, NJ 07065-0900.
Gene. 1987;57(1):47-52. doi: 10.1016/0378-1119(87)90175-2.
Two recombinant DNA plasmids were constructed with identical transcriptional and translational regulatory elements controlling expression of the bovine growth hormone (bGH) gene or the bGH gene lacking introns. Transient expression of these plasmids in cultured eukaryotic cells, monitored by assaying secretion of bGH into the culture medium, was employed to examine the relative importance of introns in the expression of this gene. The bGH gene lacking introns is expressed more efficiently than the bGH gene in avian and mammalian cells.
构建了两个重组DNA质粒，它们具有相同的转录和翻译调控元件，用于控制牛生长激素（bGH）基因或缺失内含子的bGH基因的表达。通过检测bGH分泌到培养基中的情况来监测这些质粒在培养的真核细胞中的瞬时表达，以此来研究内含子在该基因表达中的相对重要性。在禽类和哺乳动物细胞中，缺失内含子的bGH基因比完整的bGH基因表达效率更高。
