Ugurel Elif, Kucuksumer Zeynep, Eglenen Buse, Yalcin Ozlem
Clin Hemorheol Microcirc. 2017;66(2):143-155. doi: 10.3233/CH-160219.
Erythrocytes undergo irreversible morphological and biochemical changes during storage. Reduced levels of deformability have been reported for stored erythrocytes. Erythrocyte deformability is essential for healthy microcirculation.
The aim of this study is to evaluate shear stress (SS) induced improvements of erythrocyte deformability in stored blood.
Deformability changes were evaluated by applying physiological levels of SS (5 and 10 Pa) in metabolically depleted blood for 48 hours and stored blood for 35 days with citrate phosphate dextrose adenine-1 (CPDA-1). Laser diffractometry was used to measure erythrocyte deformability before and after application of SS.
Erythrocyte deformability, as a response to continuous SS, was significantly improved in metabolically depleted blood, whereas it was significantly impaired in the blood stored for 35 days with CPDA-1 (p≤0.05). The SS-induced improvements of deformability were deteriorated due to storage and relatively impaired according to the storage time. However, deformability of stored blood after exposure to mechanical stress tends to increase at low levels of shear while decreasing at high SS levels.
Impairment of erythrocyte deformability after storage may contribute to impairments in the recipient's microcirculation after blood transfusion. The period of the storage should be considered to prevent microcirculatory problems and insufficient oxygen delivery to the tissues.
红细胞在储存过程中会发生不可逆的形态和生化变化。已有报道称储存的红细胞变形性水平降低。红细胞变形性对于健康的微循环至关重要。
本研究旨在评估剪切应力（SS）对储存血液中红细胞变形性的改善作用。
通过在代谢耗尽的血液中施加生理水平的SS（5和10帕斯卡）48小时以及在含有枸橼酸盐磷酸盐葡萄糖腺嘌呤-1（CPDA-1）的条件下储存35天的血液中施加SS来评估变形性变化。在施加SS前后，使用激光衍射法测量红细胞变形性。
作为对持续SS的反应，代谢耗尽的血液中红细胞变形性显著改善，而在含有CPDA-1储存35天的血液中红细胞变形性显著受损（p≤0.05）。由于储存，SS诱导的变形性改善作用减弱，并且根据储存时间相对受损。然而，储存血液在暴露于机械应力后，其变形性在低剪切水平时倾向于增加，而在高SS水平时降低。
储存后红细胞变形性受损可能导致输血后受者微循环受损。应考虑储存时间以预防微循环问题和组织氧输送不足。
