Mudi Sonchita R, Akhter Masfida, Biswas Subrata K, Muttalib Mohammad A, Choudhury Subhagata, Rokeya Begum, Ali Liaquat
J Complement Integr Med. 2017 Mar 9;14(2). doi: 10.1515/jcim-2016-0111.
BackgroundAegle marmelos is a popular fruit plant in the Indian subcontinent, various parts of which are traditionally used against various illnesses including diabetes mellitus (DM). However, the underlying mechanisms of the antidiabetic effects of the plant are not clear, especially in type 2 DM. The present study was undertaken to investigate the effect of aqueous extracts of A. marmelos fruits (AMFE) and leaves (AMLE) on glycemic, lipidemic, insulinemic, insulin resistance and β-cell functional status of type 2 diabetic model rats. Methods An interventional study was designed using 20 type 2 diabetic rats. Type 2 DM was induced in Long Evans rats by a single intra-peritoneal injection of streptozotocin (90 mg/kg body weight) to 48 h old pups. Three months after induction of diabetes, the rats were divided into three independent groups: water-treated control group (n=6), AMLE-treated group (n=7) and AMFE-treated group (n=7). The rats were fed with extracts or water for 21 consecutive days and blood samples were collected at days 0 and 21 after an overnight fast. Data were expressed as mean±SD and analyzed by paired t-test or ANOVA as appropriate. Results There were significantly lower blood glucose values in AMLE and AMFE groups at Endpoint compared to Baseline (mmol/l, mean±SD, Baseline vs. Endpoint, 7.04±1.0 vs. 6.06±0.92; p=0.032 and 7.04±0.97 vs. 5.87±0.93; p=0.047). There were also significantly lower serum insulin levels in AMLE and AMFE groups at Endpoint compared to Baseline (µIU/mL, mean±SD, Baseline vs. Endpoint, 14.02±5.48 vs. 7.57±2.90; p=0.026 and 11.54±4.83 vs. 6.58±4.36; p=0.008). Insulin resistance (HOMA-IR) was significantly improved both in AMLE and AMFE groups at Endpoint compared to Baseline (mean±SD, Baseline vs. Endpoint, 4.22±1.68 vs. 2.05±0.90; p=0.021 and 3.69±1.79 vs. 1.69±1.61; p=0.013). However, β-cell function or lipid profile did not show any significant alteration at Endpoint compared to Baseline in AMLE and AMFE groups. Conclusions Aqueous extracts of A. marmelos leaf and fruit have hypoglycemic property which seem to be mediated by lowering of insulin resistance. These findings highlight the therapeutic potential of the extracts of A. marmelos in human type 2 DM and provides strong impetus for further studies.
背景
印度楝是印度次大陆一种常见的水果植物，其各个部位传统上被用于治疗包括糖尿病（DM）在内的各种疾病。然而，该植物抗糖尿病作用的潜在机制尚不清楚，尤其是在2型糖尿病方面。本研究旨在探讨印度楝果实水提取物（AMFE）和叶子水提取物（AMLE）对2型糖尿病模型大鼠的血糖、血脂、胰岛素水平、胰岛素抵抗及β细胞功能状态的影响。
方法
采用20只2型糖尿病大鼠进行一项干预性研究。通过对48小时龄的Long Evans幼鼠单次腹腔注射链脲佐菌素（90 mg/kg体重）诱导2型糖尿病。糖尿病诱导三个月后，将大鼠分为三个独立组：水处理对照组（n = 6）、AMLE处理组（n = 7）和AMFE处理组（n = 7）。大鼠连续21天喂食提取物或水，并在禁食过夜后的第0天和第21天采集血样。数据以均值±标准差表示，并根据情况通过配对t检验或方差分析进行分析。
结果
与基线相比，AMLE组和AMFE组在终点时的血糖值显著降低（mmol/l，均值±标准差，基线 vs. 终点，7.04±1.0 vs. 6.06±0.92；p = 0.032和7.04±0.97 vs. 5.87±0.93；p = 0.047）。与基线相比，AMLE组和AMFE组在终点时的血清胰岛素水平也显著降低（µIU/mL，均值±标准差，基线 vs. 终点，14.02±5.48 vs. 7.57±2.90；p = 0.026和11.54±4.83 vs. 6.58±4.36；p = 0.008）。与基线相比，AMLE组和AMFE组在终点时的胰岛素抵抗（HOMA-IR）均显著改善（均值±标准差，基线 vs. 终点，4.22±1.68 vs.
