Torkashvand Maryam, Gholivand Mohammad Bagher, Taherpour Avat Arman, Boochani Arash, Akhtar Arsalan
Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran.
Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran.
J Pharm Biomed Anal. 2017 May 30;139:156-164. doi: 10.1016/j.jpba.2017.02.032. Epub 2017 Mar 1.
In this paper a novel electrochemical sensor based on nickel carbide (NiC) nanoparticles as a new modifier was constructed. NiC nanoparticle was synthesized and characterized by scanning electron microscopy, X-ray diffraction and first-principles study. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) studies confirmed the electrode modification. Afterwards, the new electrode for the first time was used for interaction study between vitamin K1 and warfarin as an anticoagulant drug by differential pulse voltammetry. The adduct formation between the drug and vitamin K1 was improved by decreasing in anodic peak current of warfarin in the presence of different amounts of vitamin K1. The binding constant between warfarin and vitamin K1 was obtained by voltammetric and UV-vis and fluorescence spectroscopic methods. The molecular modeling method was also performed to explore the structural features and binding mechanism of warfarin to vitamin K1. The different aspects of modeling of vitamin K1 and warfarin and their adduct structures confirmed the adduct formation by hydrogen bonding.
本文构建了一种基于碳化镍（NiC）纳米颗粒作为新型修饰剂的新型电化学传感器。通过扫描电子显微镜、X射线衍射和第一性原理研究对NiC纳米颗粒进行了合成与表征。电化学阻抗谱（EIS）和循环伏安法（CV）研究证实了电极修饰。之后，首次使用该新型电极通过差分脉冲伏安法研究维生素K1与作为抗凝血药物的华法林之间的相互作用。在存在不同量维生素K1的情况下，华法林阳极峰电流的降低改善了药物与维生素K1之间加合物的形成。通过伏安法、紫外可见光谱法和荧光光谱法获得了华法林与维生素K1之间的结合常数。还采用分子建模方法探索了华法林与维生素K1的结构特征和结合机制。维生素K1和华法林建模及其加合物结构的不同方面证实了通过氢键形成加合物。
