Ali Tamer Awad, Mohamed Gehad Genidy, El-Sonbati Adel Zaki, Diab Mostafa Amin, Elkfass Ahmed Mohmed
Egyptian Petroleum Research Institute (EPRI), 11727, Cairo, Egypt.
Department of Chemistry, Faculty of Science, Cairo Un4ersity, 12613, Giza, Egypt.
Iran J Pharm Res. 2020 Summer;19(3):533-555. doi: 10.22037/ijpr.2019.13892.11970.
A new sensit4e and select4e modified screen printed electrodes (MSPEs) and carbon paste electrodes (MCPEs) were studied in order to determine trimbutine maleate (TM) in pure, tablets, urine, and serum samples. These sensors were embodied with multiwalled carbon nanotubes (MWCNTs) since it improved the quality of the sensors in presence of potassium tetrakis (-chlorophenyl) borate (KTpClPB) ionophore. A good Nernstian response for the constructed sensors, at optimum paste composition, was exhibited for determination of TM in concentration range of 1.5 × 10 - 1.0 × 10 and 1.0 × 10- 1.0 × 10 mol L at 25 °C with detection limit of 1.5 × 10 and 1.0 × 10 mol L for MCPE and MSPE, respect4ely. It seemed that the potential of the electrodes was independent on H in the range of 2.0-8.0, 2.0-8.5, 2.0-8.5, and 2.0-9.0 g4ing slope as 56.77 ± 1.11, 57.82 ± 0.54, 57.95 ± 0.37, and 58.99 ± 0.28 mV decade for electrodes 1, 2, 3 and 4, respect4ely. MCPEs and MSPEs gave response time about 8 and 6 s with long lifetime (more than 3 and 5 months), respect4ely. A high select4ity of sensors was observed for TM regarding to a large number of interfering species. The constructed sensors were successfully applied for determination of TM in pure form, its pharmaceutical preparations and biological fluids using standard addition, calibration, and potentiometric titration methods with high precision and accuracy. The results showed a good agreement between the proposed method and the HPLC official method.
为了测定纯品、片剂、尿液和血清样品中的马来酸曲美布汀（TM），研究了一种新型灵敏且选择性的修饰丝网印刷电极（MSPEs）和碳糊电极（MCPEs）。这些传感器采用了多壁碳纳米管（MWCNTs），因为在四（-氯苯基）硼酸钾（KTpClPB）离子载体存在的情况下，它提高了传感器的性能。在最佳糊剂组成下，构建的传感器对TM具有良好的能斯特响应，在25℃下，TM的测定浓度范围为1.5×10 - 1.0×10和1.0×10 - 1.0×10 mol/L，MCPE和MSPE的检测限分别为1.5×10和1.0×10 mol/L。电极的电位在2.0 - 8.0、2.0 - 8.5、2.0 - 8.5和2.0 - 9.0范围内似乎与H无关，电极1、2、3和4的斜率分别为56.77±1.11、57.82±0.54、57.95±0.37和58.99±0.28 mV/decade。MCPEs和MSPEs的响应时间分别约为8秒和6秒，寿命较长（分别超过3个月和5个月）。对于大量干扰物质，传感器对TM具有高选择性。使用标准加入法、校准法和电位滴定法，构建的传感器成功应用于纯品、其药物制剂和生物流体中TM的测定，具有高精度和准确性。结果表明，所提出的方法与HPLC法定方法之间具有良好的一致性。
