采用基于蛋白A-硅胶基质的高效液相亲和色谱法（HPLAC）对循环免疫复合物进行分析。

Profiling of circulating immune complexes by high performance liquid affinity chromatography (HPLAC) on a protein A-silica matrix.

作者信息

Ohlson S, Niss U

机构信息

Perstorp Biolytica AB, Lund, Sweden.

出版信息

J Immunol Methods. 1988 Feb 10;106(2):225-9. doi: 10.1016/0022-1759(88)90201-3.

DOI:10.1016/0022-1759(88)90201-3

PMID:2828476

Abstract

Protein A, a cell wall protein found in bacterial strains of Staphylococcus aureus, has been extensively used in the analysis and purification of immunoglobulins and immune complexes. By binding protein A to microparticulate silica (high performance liquid affinity chromatography, HPLAC), a rapid and efficient chromatographic system was obtained for the separation and analysis of circulating immune complexes. The method was applied to the separation of artificial immune complexes as well as to plasma samples from patients with immune complex associated diseases such as SLE and RA. It was possible to distinguish certain subpopulations of circulating immune complexes by performing pH gradients on the protein A silica HPLAC column.

摘要

蛋白A是在金黄色葡萄球菌菌株中发现的一种细胞壁蛋白，已被广泛用于免疫球蛋白和免疫复合物的分析与纯化。通过将蛋白A与微粒硅胶结合（高效液相亲和色谱法，HPLAC），获得了一种用于分离和分析循环免疫复合物的快速高效色谱系统。该方法应用于人工免疫复合物的分离以及来自患有免疫复合物相关疾病（如系统性红斑狼疮和类风湿关节炎）患者的血浆样本。通过在蛋白A硅胶HPLAC柱上进行pH梯度，可以区分循环免疫复合物的某些亚群。