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一种替代性阴离子生物可持续抗真菌剂:对其作用于真菌细胞膜方式的研究。

An alternative anionic bio-sustainable anti-fungal agent: Investigation of its mode of action on the fungal cell membrane.

作者信息

Stenbæk Jonas, Löf David, Falkman Peter, Jensen Bo, Cárdenas Marité

机构信息

Section of Microbiology, University of Copenhagen, Copenhagen, Denmark; Danish Technological Institute, Wood and Biomaterials, Gregersensvej 3, 2630 Taastrup, Denmark.

Perstorp AB, Industriparken, 284 91 Perstorp, Sweden.

出版信息

J Colloid Interface Sci. 2017 Jul 1;497:242-248. doi: 10.1016/j.jcis.2017.03.018. Epub 2017 Mar 4.

DOI:10.1016/j.jcis.2017.03.018
PMID:28285052
Abstract

The potential of a lactylate (the sodium caproyl lactylate or C10 lactylate), a typical food grade emulsifier, as an anionic environmental friendly anti-fungal additive was tested in growth medium and formulated in a protective coating for exterior wood. Different laboratory growth tests on the blue stain fungus Aureobasidium pullulans were performed and its interactions on a model fungal cell membrane were studied. Promising short term anti-fungal effects in growth tests were observed, although significant but less dramatic effects took place in coating test on wood panels. Scanning electron microscope analysis shows clear differences in the amount of fungal slime on the mycelium of Aureobasidium pullulans when the fungus was exposed of C10 lactylate. This could indicate an effect on the pullulan and melanin production by the fungus. Moreover, the interaction studies on model fungal cell membranes show that C10 lactylate affects the phospholipid bilayer in a similar manner to other negative charged detergents.

摘要

测试了一种典型的食品级乳化剂乳酸酯(己酰乳酸钠或C10乳酸酯)作为一种阴离子型环保抗真菌添加剂在生长培养基中的潜力,并将其配制在外部木材的防护涂层中。对青霉属出芽短梗霉菌进行了不同的实验室生长测试,并研究了其在模型真菌细胞膜上的相互作用。在生长测试中观察到了有前景的短期抗真菌效果,尽管在木板涂层测试中也有显著但不太明显的效果。扫描电子显微镜分析表明,当青霉属出芽短梗霉菌暴露于C10乳酸酯时,其菌丝体上真菌黏液的量有明显差异。这可能表明对该真菌的支链淀粉和黑色素产生有影响。此外,对模型真菌细胞膜的相互作用研究表明,C10乳酸酯对磷脂双层的影响与其他带负电荷的去污剂类似。

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