Department of Chemical and Process Engineering, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.
Department of Chemical and Process Engineering, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.
Bioresour Technol. 2017 Jun;233:296-304. doi: 10.1016/j.biortech.2017.02.110. Epub 2017 Mar 1.
Calcium carbonate was evaluated as a replacement for the base during the fermentation of glycerol by a highly productive strain of 1,3-propanediol (PDO), viz., Clostridium butyricum JKT37. Due to its high specific growth rate (µ=0.53h), 40g/L of glycerol was completely converted into 19.6g/L of PDO in merely 7h of batch fermentation, leaving only acetate and butyrate as the by-products. The accumulation of these volatile fatty acids was circumvented with the addition of calcium carbonate as the pH neutraliser before the fermentation was inoculated. An optimal amount of 15g/L of calcium carbonate was statistically determined from screening with various glycerol concentrations (20-120g/L). By substituting potassium hydroxide with calcium carbonate as the pH neutraliser for fermentation in a bioreactor, a similar yield (Y=0.6mol/mol) with a constant pH was achieved at the end of the fermentation.
碳酸钙被评估为一种替代物，用于由高产 1,3-丙二醇（PDO）菌株丁酸梭菌 JKT37 发酵甘油的过程中的碱。由于其高比生长速率（µ=0.53h），在批式发酵仅 7 小时内，40g/L 的甘油完全转化为 19.6g/L 的 PDO，仅留下乙酸盐和丁酸盐作为副产物。在发酵接种之前，通过添加碳酸钙作为 pH 中和剂来避免这些挥发性脂肪酸的积累。通过用不同浓度的甘油（20-120g/L）进行筛选，统计确定了最佳用量为 15g/L 的碳酸钙。通过用碳酸钙代替氢氧化钾作为 pH 中和剂在生物反应器中进行发酵，在发酵结束时实现了类似的产率（Y=0.6mol/mol）和恒定的 pH 值。
