CHAF1A在上皮性卵巢癌中的过表达可促进细胞增殖并抑制细胞凋亡。
Over-expression of CHAF1A in Epithelial Ovarian Cancer can promote cell proliferation and inhibit cell apoptosis.
作者信息
Xia Dandan, Yang Xiaoming, Liu Wenxue, Shen Fangqian, Pan Jufang, Lin Yu, Du Na, Sun Yunyan, Xi Xiaowei
机构信息
Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China.
Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China.
出版信息
Biochem Biophys Res Commun. 2017 Apr 22;486(1):191-197. doi: 10.1016/j.bbrc.2017.03.026. Epub 2017 Mar 9.
Chromatin Assembly Factor 1, subunit A (CHAF1A) can regulate cell proliferation, DNA repair and epigenetic changes in embryonic stem cells and it has been reported that over-expression of CHAF1A is associated with several human diseases including cancer. However, the expression and function of CHAF1A in Epithelial Ovarian Cancer (EOC) are rarely reported at present. In this study, we found that the positive staining of CHAF1A in EOC was higher than that in normal tissues and over-expression of CHAF1A was strongly associated with cancer stage and lymph node metastasis. Knockdown of CHAF1A by siRNA in EOC inhibited cell proliferation, reduced colony formation, caused G0/G1 phase arrest and promoted cell apoptosis. Taken together, the high expression of CHAF1A promotes cell proliferation and inhibits cell apoptosis and CHAF1A may be developed as a prognosis biomarker and potential therapeutic target of EOC.
染色质组装因子1亚基A（CHAF1A）可调节胚胎干细胞的细胞增殖、DNA修复和表观遗传变化，并且据报道CHAF1A的过表达与包括癌症在内的多种人类疾病相关。然而，目前关于CHAF1A在上皮性卵巢癌（EOC）中的表达和功能鲜有报道。在本研究中，我们发现EOC中CHAF1A的阳性染色高于正常组织，且CHAF1A的过表达与癌症分期和淋巴结转移密切相关。通过小干扰RNA（siRNA）敲低EOC中的CHAF1A可抑制细胞增殖、减少集落形成、导致G0/G1期阻滞并促进细胞凋亡。综上所述，CHAF1A的高表达促进细胞增殖并抑制细胞凋亡，CHAF1A可能被开发成为EOC的预后生物标志物和潜在治疗靶点。
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