a Department of Experimental Medicine , Sapienza University of Rome , Rome , Italy.
b Cardiovascular and Anesthesia-Intensive Care and Department of Cardiac and Heart Transplantation San Camillo-Forlanini Hospital , Rome , Italy.
Platelets. 2017 Nov;28(7):728-730. doi: 10.1080/09537104.2017.1293803. Epub 2017 Mar 13.
Heparin-induced thrombocytopenia (HIT) is a thrombotic complication of heparin therapy. The most used functional method for HIT diagnosis is serotonin release assay (SRA). A different functional method based on ATP release with luciferin/luciferase long-life and stable luminescent signal is used here, which is shown to be comparable for accuracy with SRA in both negative (patients 4Ts ≤3, and negative for both anti-PF4/heparin immunoassay and SRA) and positive (4Ts >3, and positive for both PF4/heparin antibodies and SRA) patients. Our results show that ATP release is higher in washed platelets activated by sera from positive patients than in platelets activated by sera from negative patients. In conclusion, we demonstrate that ATP release assay is a valid alternative method to SRA for the identification of pathogenic anti-PF4/heparin antibodies.
肝素诱导的血小板减少症（HIT）是肝素治疗的一种血栓并发症。最常用于 HIT 诊断的功能方法是血清素释放测定（SRA）。这里使用了一种基于 ATP 释放的不同功能方法，该方法使用长寿命和稳定发光信号的荧光素/荧光素酶，其在阴性（患者 4Ts ≤3，且抗 PF4/肝素免疫测定和 SRA 均为阴性）和阳性（4Ts >3，且 PF4/肝素抗体和 SRA 均为阳性）患者中的准确性与 SRA 相当。我们的结果表明，由阳性患者的血清激活的洗涤血小板释放的 ATP 高于由阴性患者的血清激活的血小板释放的 ATP。总之，我们证明了 ATP 释放测定是 SRA 的一种有效替代方法，可用于鉴定致病性抗 PF4/肝素抗体。
